Coordinate expression of secretory phospholipase A2 and cyclooxygenase-2 in activated human keratinocytes
1 Department of Dermatology, University of Rochester Medical Center, Rochester, New York 14642; and 2 Drug Discovery Enterprises, Hughes Institute, St. Louis, Missouri 55113 PGE 2 levels are altered in human epidermis after in vivo wounding; however, mechanisms modulating PGE 2 production in activ...
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Published in | American Journal of Physiology: Cell Physiology Vol. 278; no. 4; p. C822 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
01.04.2000
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Online Access | Get full text |
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Summary: | 1 Department of Dermatology, University of
Rochester Medical Center, Rochester, New York 14642; and
2 Drug Discovery Enterprises, Hughes Institute,
St. Louis, Missouri 55113
PGE 2 levels are altered in human epidermis
after in vivo wounding; however, mechanisms modulating
PGE 2 production in activated keratinocytes are unclear. In
previous studies, we showed that PGE 2 is a growth-promoting
autacoid in human primary keratinocyte cultures, and its production is
modulated by plating density, suggesting that regulated
PGE 2 synthesis is an important component of wound healing.
Here, we examine the role of phospholipase A 2 (PLA 2 ) and cyclooxygenase (COX) enzymes in modulation of
PGE 2 production. We report that the increased
PGE 2 production that occurs in keratinocytes grown in
nonconfluent conditions is also observed after in vitro wounding,
indicating that similar mechanisms are involved. This increase was
associated with coordinate upregulation of both COX-2 and secretory
PLA 2 (sPLA 2 ) proteins. Increased
sPLA 2 activity was also observed. By RT-PCR, we identified
the presence of type IIA and type V sPLA 2 , along with the
M-type sPLA 2 receptor. Thus the coordinate expression of
sPLA 2 and COX-2 may be responsible for the increased
prostaglandin synthesis in activated keratinocytes during wound repair.
wound healing; primary human keratinocytes; arachidonic acid; prostaglandin E 2 ; secretory phospholipase A 2
receptor |
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ISSN: | 0363-6143 1522-1563 |
DOI: | 10.1152/ajpcell.2000.278.4.c822 |