Characterization of a sperm-specific nuclear autoantigenic protein. II. Expression and localization in the testis
The testis- and sperm-specific nuclear autoantigenic protein, NASP, has a 2.5-kb mRNA that encodes a protein of molecular weight 73,533 and has several structural features of nuclear proteins. To further characterize NASP and confirm the structural predictions that NASP was a nuclear protein, specif...
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Published in | Biology of reproduction Vol. 43; no. 4; pp. 569 - 578 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Society for the Study of Reproduction
01.10.1990
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Subjects | |
Online Access | Get full text |
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Summary: | The testis- and sperm-specific nuclear autoantigenic protein, NASP, has a 2.5-kb mRNA that encodes a protein of molecular
weight 73,533 and has several structural features of nuclear proteins. To further characterize NASP and confirm the structural
predictions that NASP was a nuclear protein, specific immunostaining using a specific anti-recombinant protein antibody and
in situ hybridization with a cDNA were used. In testis sections, NASP was first detected in the nuclear area of primary spermatocytes.
During the subsequent meiotic divisions, NASP was partitioned into the cytoplasm and then reassociated with the reforming
nucleus. No antibody labeling was associated with the chromatin. During spermiogenesis, NASP became restricted to the post-acrosomal
region of the spermatozoon, although some labeling appeared in residual bodies and subsequently in the tubule lumen. NASP
was not found in somatic cells. The detection of NASP mRNA transcripts in primary spermatocytes by in situ hybridization supported
the immunolocalization results and indicated that NASP expression was under transcriptional control. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod43.4.569 |