Reduction-Mediated Technetium-99m Labeling of Monoclonal Antibodies
A simple and generally applicable method for labeling antibodies with technetium-99m (99mTc) is described. Following reduction of intrinsic disulphide bonds, the antibody is labeled with 99mTc in the presence of a weak competing ligand methylene diphosphonate. High labeling efficiencies (greater tha...
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| Published in | The Journal of nuclear medicine (1978) Vol. 31; no. 5; pp. 692 - 697 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Reston, VA
Soc Nuclear Med
01.05.1990
Society of Nuclear Medicine |
| Subjects | |
| Online Access | Get full text |
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| Abstract | A simple and generally applicable method for labeling antibodies with technetium-99m (99mTc) is described. Following reduction of intrinsic disulphide bonds, the antibody is labeled with 99mTc in the presence of a weak competing ligand methylene diphosphonate. High labeling efficiencies (greater than 97%), in a final labeling step taking only a few minutes, can be routinely obtained with high in-vitro stability over 24 hr. No effect upon antibody reactivity is seen. |
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| AbstractList | A simple and generally applicable method for labeling antibodies with technetium-99m ({sup 99m}Tc) is described. Following reduction of intrinsic disulphide bonds, the antibody is labeled with {sup 99m}Tc in the presence of a weak competing ligand methylene diphosphonate. High labeling efficiencies (greater than 97%), in a final labeling step taking only a few minutes, can be routinely obtained with high in-vitro stability over 24 hr. No effect upon antibody reactivity is seen. A simple and generally applicable method for labeling antibodies with technetium-99m (99mTc) is described. Following reduction of intrinsic disulphide bonds, the antibody is labeled with 99mTc in the presence of a weak competing ligand methylene diphosphonate. High labeling efficiencies (greater than 97%), in a final labeling step taking only a few minutes, can be routinely obtained with high in-vitro stability over 24 hr. No effect upon antibody reactivity is seen. |
| Author | Mather, Stephen J Ellison, David |
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| Keywords | Nuclear medicine Technetium Monoclonal antibody Radiolabelling Radiopharmaceuticals |
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| Snippet | A simple and generally applicable method for labeling antibodies with technetium-99m (99mTc) is described. Following reduction of intrinsic disulphide bonds,... A simple and generally applicable method for labeling antibodies with technetium-99m ({sup 99m}Tc) is described. Following reduction of intrinsic disulphide... |
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| SubjectTerms | 550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-) ANIMALS ANTIBODIES Antibodies, Monoclonal - pharmacokinetics BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences DISTRIBUTION HOURS LIVING RADIOISOTOPES INTERMEDIATE MASS NUCLEI Investigative techniques, diagnostic techniques (general aspects) ISOMERIC NUCLEI ISOMERIC TRANSITION ISOTOPES Isotope Labeling - methods ISOTOPES LABELLING MAMMALS Medical sciences MICE MONOCLONAL ANTIBODIES NUCLEI ODD-EVEN NUCLEI RADIOISOTOPES RADIOLOGY AND NUCLEAR MEDICINE RODENTS STABILITY Technetium TECHNETIUM 99 TECHNETIUM ISOTOPES TISSUE DISTRIBUTION VERTEBRATES YEARS LIVING RADIOISOTOPES 550601 -- Medicine-- Unsealed Radionuclides in Diagnostics |
| Title | Reduction-Mediated Technetium-99m Labeling of Monoclonal Antibodies |
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