Reduction-Mediated Technetium-99m Labeling of Monoclonal Antibodies

• Published in: The Journal of nuclear medicine (1978) Vol. 31; no. 5; pp. 692 - 697
• Main Authors: Mather, Stephen J, Ellison, David
• Format: Journal Article
• Language: English
• Published: Reston, VA Soc Nuclear Med 01.05.1990; Society of Nuclear Medicine
• Subjects: 550604 > Medicine > Unsealed Radionuclides in Therapy > (1980-); ANIMALS; ANTIBODIES; Antibodies, Monoclonal - pharmacokinetics; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; Biological and medical sciences; DISTRIBUTION; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; Investigative techniques, diagnostic techniques (general aspects); ISOMERIC NUCLEI; ISOMERIC TRANSITION ISOTOPES; Isotope Labeling - methods; ISOTOPES; LABELLING; MAMMALS; Medical sciences; MICE; MONOCLONAL ANTIBODIES; NUCLEI; ODD-EVEN NUCLEI; RADIOISOTOPES; RADIOLOGY AND NUCLEAR MEDICINE; RODENTS; STABILITY; Technetium; TECHNETIUM 99; TECHNETIUM ISOTOPES; TISSUE DISTRIBUTION; VERTEBRATES; YEARS LIVING RADIOISOTOPES 550601 > Medicine > Unsealed Radionuclides in Diagnostics; Nuclear medicine; Technetium; Monoclonal antibody; Radiolabelling; Radiopharmaceuticals
• ISSN: 0161-5505; 1535-5667

A simple and generally applicable method for labeling antibodies with technetium-99m (99mTc) is described. Following reduction of intrinsic disulphide bonds, the antibody is labeled with 99mTc in the presence of a weak competing ligand methylene diphosphonate. High labeling efficiencies (greater than 97%), in a final labeling step taking only a few minutes, can be routinely obtained with high in-vitro stability over 24 hr. No effect upon antibody reactivity is seen.