A guanine nucleotide-sensitive, glucagon-stimulated adenylyl cyclase in Candida albicans: effect of glucagon on cell morphology

GTP, GTP-gamma-S and Gpp(NH)p produced a significant activation of Mg2(+)-dependent adenylyl cyclase in permeabilized cells of Candida albicans. This activation was inhibited by GDP-beta-S. Maximal stimulation (4-6 fold) was obtained when Mg2+ and GTP-gamma-S were added during permeabilization. The...

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Published inBiochemical and biophysical research communications Vol. 167; no. 3; pp. 1177 - 1183
Main Authors Paveto, C, Egidy, G, Galvagno, M A, Passeron, S
Format Journal Article
LanguageEnglish
Published United States Elsevier 30.03.1990
Subjects
Adenylyl Cyclases - metabolism
Candida albicans - cytology
Candida albicans - drug effects
Candida albicans - enzymology
Enzyme Activation
Glucagon - pharmacology
Guanosine 5'-O-(3-Thiotriphosphate)
Guanosine Triphosphate - analogs & derivatives
Guanosine Triphosphate - pharmacology
Guanylyl Imidodiphosphate - pharmacology
Kinetics
Life Sciences
Magnesium - pharmacology
Thionucleotides - pharmacology
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Summary:GTP, GTP-gamma-S and Gpp(NH)p produced a significant activation of Mg2(+)-dependent adenylyl cyclase in permeabilized cells of Candida albicans. This activation was inhibited by GDP-beta-S. Maximal stimulation (4-6 fold) was obtained when Mg2+ and GTP-gamma-S were added during permeabilization. The guanine nucleotide-stimulated activity could be further activated by glucagon in a dose dependent manner being the maximal stimulation (4-5 fold) attained at 10(-6) M glucagon. Addition of the hormone to yeast cells incubated under conditions conducive to produce germ tubes blocked hyphal development and promoted multibudded cell morphology.
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(90)90647-6