Skeletal muscle intracellular PO2 assessed by myoglobin desaturation: response to graded exercise

• Published in: Journal of applied physiology (1985) Vol. 91; no. 6; pp. 2679 - 2685
• Main Authors: Richardson, R. S, Newcomer, S. C, Noyszewski, E. A
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Physiological Soc 01.12.2001; American Physiological Society
• Subjects: Biological and medical sciences; Exercise; Fundamental and applied biological sciences. Psychology; Muscular system; Oxygen; Striated muscle. Tendons; Vertebrates: osteoarticular system, musculoskeletal system; Knee; Human; Myoglobin; Oxygen; Biological transport; Lower limb; Striated muscle; Maximal physical exercise; Extension; Intracellular; Oxygen pressure; Ergometry; Desaturation
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/jappl.2001.91.6.2679

1  Department of Medicine, University of California San Diego, La Jolla, California 92093; and 2  Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6021 The relationship between skeletal muscle intracellular P O 2 (iP O 2 ) and progressive muscular work has important implications for the understanding of O 2 transport and utilization. Presently there is debate as to whether iP O 2 falls progressively with increasing O 2 demand or reaches a plateau from moderate to maximal metabolic demand. Thus, using 1 H magnetic resonance spectroscopy of myoglobin (Mb), we studied cellular oxygenation during progressive single-leg knee extensor exercise from unweighted to 100% of maximal work rate in six active human subjects. In all subjects, the Mb peak at 73 ppm was not visible at rest, whereas the peak was small or indistinguishable from the noise in the majority of subjects during progressive exercise from unweighted to 50-60% of maximum work rate. In contrast, beyond this exercise intensity, a Mb peak of consistent magnitude was discernible in all subjects. When a Mb half saturation of 3.2 Torr was used, the calculated skeletal muscle P O 2 was variable before 60% of maximum work rate but in general was relatively high (>18 Torr, the measurable P O 2 with the poorest signal-to-noise ratio, in the majority of cases), whereas beyond this exercise intensity iP O 2 fell to a relatively uniform and invariant level of 3.8 ± 0.5 Torr across all subjects. These results do not support the concept of a progressive linear fall in iP O 2 across increasing work rates. Instead, this study documents variable but relatively high iP O 2 from rest to moderate exercise and again confirms that from 50-60% of maximum work rate iP O 2 reaches a plateau that is then invariant with increasing work rate. oxygen; work rate; diffusion; oxygen transport