Dethiosulfovibrio russensis sp. nov., Dethiosulfovibrio marinus sp. nov. and Dethiosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments
AV Surkov, GA Dubinina, AM Lysenko, FO Glockner and J Kuever Laboratory of Ecology and Geochemical Activity of Microorganisms, Institute of Microbiology of Russian Academy of Sciences, Moscow, Russia Four strains of strictly anaerobic, sulfur- and thiosulfate-reducing bacteria, SR12(T), SR13, SR15(T...
Saved in:
Published in | International journal of systematic and evolutionary microbiology Vol. 51; no. 2; pp. 327 - 337 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English Russian |
Published |
Reading
Soc General Microbiol
01.03.2001
Society for General Microbiology |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | AV Surkov, GA Dubinina, AM Lysenko, FO Glockner and J Kuever
Laboratory of Ecology and Geochemical Activity of Microorganisms, Institute of Microbiology of Russian Academy of Sciences, Moscow, Russia
Four strains of strictly anaerobic, sulfur- and thiosulfate-reducing
bacteria, SR12(T), SR13, SR15(T) and WS100(T), were isolated from
'Thiodendron' sulfur mats obtained from different saline environments. All
isolates were motile, Gram-negative, non-spore-forming curved rods with
pointed or rounded ends. The sizes of cells varied from 0.9 x 3--5 microm
for strains SR12(T), SR13 and SR15(T) to 0.9 x 4--8 microm for strain
WS100(T). All strains could form long spiral filamentous cells up to
70--110 microm during the early stage of growth. All strains were motile by
a tumbling movement and possessed lateral flagella arranged at the concave
side of cells. Incomplete cross-septa were distinctive features of all
strains. Growth occurred at temperatures of 10--40 degrees C with an
optimum at 28 degrees C. The pH limits for growth were 5.5 to 8.0, with
optimal growth at pH 6.5--7.0. All isolates were obligately anaerobic and
slightly halophilic and grew in media containing 0.5--5% NaCl with an
optimum at 2% NaCl. All strains were chemoorganoheterotrophic, having a
fermentative type of metabolism and utilized proteins, peptides, amino
acids and some organic acids, but not sugars, fatty acids or alcohols. Some
organic substrates (isoleucine, valine, alanine, glutamate) were utilized
only by strain SR12(T) in the presence of sulfur or thiosulfate.
Fermentation of citrate yielded mainly acetate, CO(2) and H(2). Sulfur and
thiosulfate were reduced to hydrogen sulfide during the fermentation of
organic substances, which increased cell yields and growth rates. Sulfate,
sulfite, fumarate, nitrate, Fe(2)O(3), MnO(2), DMSO and elemental selenium
were not used as electron acceptors by these strains. The G+C contents of
the DNA were 51 mol% for strains SR12(T), SR13 and SR15(T) and 52 mol% for
strain WS100(T). Based on morphological, physiological and phylogenetic
similarities, all four isolates could be assigned to three new species of
the genus Dethiosulfovibrio, named Dethiosulfovibrio russensis (type strain
DSM 12538(T)), Dethiosulfovibrio marinus (type strain DSM 12537(T)) and
Dethiosulfovibrio acidaminovorans (type strain DSM 12590(T)). |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1466-5026 1466-5034 |
DOI: | 10.1099/00207713-51-2-327 |