Genetic crosses between Ectocarpus strains

• Published in: Cold Spring Harbor protocols Vol. 2012; no. 2; p. 262
• Main Authors: Coelho, Susana M, Scornet, Delphine, Rousvoal, Sylvie, Peters, Nick, Dartevelle, Laurence, Peters, Akira F, Cock, J Mark
• Format: Journal Article
• Language: English
• Published: United States 01.02.2012
• Subjects: Crosses, Genetic; Diploidy; Genetics, Microbial - methods; Haploidy; Phaeophyceae - cytology; Phaeophyceae - genetics; Recombination, Genetic

This article describes a procedure for conducting crosses between different strains of Ectocarpus. Crossing gametophytes to obtain the sporophyte generation is the most technically challenging stage of this process because diploid sporophytes have to be distinguished from the haploid partheno-sporop...