Genetic crosses between Ectocarpus strains

This article describes a procedure for conducting crosses between different strains of Ectocarpus. Crossing gametophytes to obtain the sporophyte generation is the most technically challenging stage of this process because diploid sporophytes have to be distinguished from the haploid partheno-sporop...

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Bibliographic Details
Published inCold Spring Harbor protocols Vol. 2012; no. 2; p. 262
Main Authors Coelho, Susana M, Scornet, Delphine, Rousvoal, Sylvie, Peters, Nick, Dartevelle, Laurence, Peters, Akira F, Cock, J Mark
Format Journal Article
LanguageEnglish
Published United States 01.02.2012
Subjects
Crosses, Genetic
Diploidy
Genetics, Microbial - methods
Haploidy
Phaeophyceae - cytology
Phaeophyceae - genetics
Recombination, Genetic
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Summary:This article describes a procedure for conducting crosses between different strains of Ectocarpus. Crossing gametophytes to obtain the sporophyte generation is the most technically challenging stage of this process because diploid sporophytes have to be distinguished from the haploid partheno-sporophytes that result from the parthenogenetic germination of unfused gametes. This requires careful monitoring of the progeny of the genetic cross until they have developed sufficiently to be transferred to a separate Petri dish. Genetic crosses allow several classical genetic methodologies to be applied in Ectocarpus, including allelic complementation tests, backcrosses, combination of different genetic mutations, and outcrosses to create mapping populations.
ISSN:1559-6095
DOI:10.1101/pdb.prot067942