Bafilomycin A1 inhibits rhinovirus infection in human airway epithelium: effects on endosome and ICAM-1
1 Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, Sendai 980-8574; 3 Virus Center, Clinical Research Division, Sendai National Hospital, Sendai 983-0045; and 2 Department of Pulmonary Medicine, Institute of Clinical Medicine, University of Tsukuba, Tsukuba...
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Published in | American journal of physiology. Lung cellular and molecular physiology Vol. 280; no. 6; p. 1115 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.06.2001
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Online Access | Get full text |
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Summary: | 1 Department of Geriatric and Respiratory Medicine, Tohoku
University School of Medicine, Sendai 980-8574;
3 Virus Center, Clinical Research Division, Sendai National
Hospital, Sendai 983-0045; and 2 Department of
Pulmonary Medicine, Institute of Clinical Medicine,
University of Tsukuba, Tsukuba 305-8575, Japan
To examine
the effects of bafilomycin A 1 , a blocker of vacuolar
H + -ATPase, on rhinovirus (RV) infection in the airway
epithelium, primary cultures of human tracheal epithelial cells were
infected with RV14. Viral infection was confirmed by showing that viral RNA in the infected cells and the viral titers in the supernatants of
infected cells increased with time. RV14 infection upregulated the
production of cytokines and mRNA of intercellular adhesion molecule
(ICAM)-1 in epithelial cells. Bafilomycin A 1 reduced the
viral titers of RV14 and inhibited the production of cytokines and
ICAM-1 before and after RV14 infection. Bafilomycin A 1
reduced susceptibility of epithelial cells to RV14 infection. RV14
increased activated nuclear factor- B in the cells, and bafilomycin
A 1 reduced the activated nuclear factor- B. Bafilomycin
A 1 decreased the number of acidic endosomes in the
epithelial cells. These results suggest that bafilomycin A 1
may inhibit infection by RV14 by not only blocking RV RNA entry into
the endosomes but also reducing ICAM-1 expression in the epithelial
cells. Bafilomycin A 1 may therefore modulate airway
inflammation after RV infection.
intercellular adhesion molecule-1; asthma; common cold; airway
inflammation; vacuolar adenosine 5'-triphosphatase |
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ISSN: | 1040-0605 1522-1504 |
DOI: | 10.1152/ajplung.2001.280.6.l1115 |