Reliability of in vitro microleakage tests: a literature review

• Published in: The journal of adhesive dentistry Vol. 3; no. 4; p. 295
• Main Authors: Raskin, A, D'Hoore, W, Gonthier, S, Degrange, M, Déjou, J
• Format: Journal Article
• Language: English
• Published: Germany 2001
• Subjects: Coloring Agents; Dental Cavity Preparation; Dental Leakage - classification; Dental Materials - chemistry; Dental Restoration, Permanent; Evaluation Studies as Topic; Humans; Immersion; Materials Testing; Reproducibility of Results; Rosaniline Dyes; Statistics, Nonparametric; Stress, Mechanical; Temperature; Thermodynamics; Time Factors; Water
• ISSN: 1461-5185; 1757-9988
• DOI: 10.3290/j.jad.a7482

The literature contains conflicting data about in vitro microleakage evaluations and their usefulness and reliability. No standardization has yet been established. Here we consider features of published studies that might affect the results of the in vitro microleakage tests. We reviewed 144 in vitro microleakage studies, published in 14 international reviews between 1992 and 1998, which comprised 917 sets or groups of experiments. The published studies were entered in a database and compared using selected literature criteria: sample, cavities, restoration procedures, thermocycling and mechanical cycling, evaluation method. The methods employed vary widely. The most frequent methodological choices (%) were (1) specimen storage after extraction: duration (unspecified, 59.2), medium (distilled or deionized water, 33.8), temperature (unspecified, 52.2), additives (none, 47.0); (2) aging method (79.1): duration before aging (< 24 h, 35.9); medium and temperature of storage before aging (distilled or deionized water, 26.8; 37 degrees C, 54.3); (3) medium of cycling (tap water, 50.5), number of cycles ([250-500], 34.6), number of baths (2, 84.0), bath temperature (5 degrees C to 55 degrees C, 60.6), immersion dwell time (30 s, 44.3); (4) tracer: type (basic fuchsin, 40.7), time of immersion (after thermocycling and/or mechanical cycles, 64.1), immersion duration (basic fuchsin: 24 h, 59.5); assessment of dye penetration of sections (91.7): direction (perpendicular, 88.5), number (1, 47.1). The great variability in the methods used in these 144 studies prevented meta-analysis and comparison of the results, thus reducing the value of these methods.