Retinal vessels: proliferation of endothelium in vitro

Tissue culture of retinal vessels from fetal calf eyes produced colonies of endothelium, pericytes, and smooth muscle. Identification of endothelial cells was based upon culture morphology, [3H]thymidine labeling of isolated vessels, and factor VIII immunofluorescence. Thimerosal added to the cultur...

Full description

Saved in:
Bibliographic Details
Published inInvestigative ophthalmology & visual science Vol. 18; no. 11; pp. 1191 - 1195
Main Authors Buzney, SM, Massicotte, SJ
Format Journal Article
LanguageEnglish
Published United States ARVO 01.11.1979
Subjects
Animals
Cattle
Cell Division
Culture Techniques
Endothelium - cytology
Endothelium - ultrastructure
Muscle, Smooth, Vascular - cytology
Retinal Vessels - cytology
Retinal Vessels - ultrastructure
Online AccessGet full text

Cover

More Information
Summary:Tissue culture of retinal vessels from fetal calf eyes produced colonies of endothelium, pericytes, and smooth muscle. Identification of endothelial cells was based upon culture morphology, [3H]thymidine labeling of isolated vessels, and factor VIII immunofluorescence. Thimerosal added to the culture medium destroyed pericytes and muscle cells, leaving only endothelial cell colonies in the primary cultures. This tissue culture system may be useful in the study of retinal vascular cell biochemistry and pathophysiology.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0146-0404
1552-5783