Effect of Hypoxia on the Accumulation of Technetium-99m-Glucarate and Technetium-99m-Gluconate by Chinese Hamster Ovary Cells In Vitro

• Published in: The Journal of nuclear medicine (1978) Vol. 34; no. 2; pp. 242 - 245
• Main Authors: Ballinger, James R, Cowan, David S. M, Boxen, Izzie, Zhang, Zong Mei, Rauth, Andrew M
• Format: Journal Article
• Language: English
• Published: United States Soc Nuclear Med 01.02.1993
• Subjects: Animals; Cell Hypoxia; Cells, Cultured; Cricetinae; Cricetulus; Female; Fructose - pharmacology; Glucaric Acid - analogs & derivatives; Glucaric Acid - pharmacokinetics; Gluconates - pharmacokinetics; Organotechnetium Compounds - pharmacokinetics; Ovary; Sodium Pertechnetate Tc 99m - pharmacokinetics; Technetium Tc 99m Pentetate - pharmacokinetics
• ISSN: 0161-5505; 1535-5667

The accumulation of 99mTc-glucarate, an agent recently reported to localize in acutely infarcted myocardium, zones of acute cerebral injury and tumors, has been compared with 99mTc-gluconate in an in vitro system of cultured Chinese hamster ovary fibroblasts. The effects on accumulation of hypoxia and competition with fructose have been studied. Both labeled glucose analogs showed a two- to threefold enhanced accumulation in hypoxic cells relative to aerobic cells. No such enhanced accumulation under hypoxia was observed for the nonsugar tracers pertechnetate and 99mTc-DTPA. The presence of 20 mM fructose reduced the accumulation of 99mTc-glucarate by 30% (p = 0.067) and 99mTc-gluconate by 40% (p < 0.05) in hypoxic cells, but had no significant effect in aerobic cells. These results suggest that both compounds at least partially share a common mechanism of uptake and/or accumulation with fructose.