Structural Studies on the Murine Ia Alloantigens: VI. Evidence That Both Subunits of the I-A Alloantigen Are Encoded by the I-A Subregion

The alpha and beta subunits of the murine I-A alloantigens from several H-2 haplotypes were examined by comparative tryptic peptide mapping by using double label (3H and 14C) techniques. Significant structural variation between alleles was detected in both subunits. Tryptic digests of the alpha poly...

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Published inThe Journal of immunology (1950) Vol. 123; no. 6; pp. 2799 - 2803
Main Authors Cook, Richard G, Capra, J. Donald, Bednarczyk, John L, Uhr, Jonathan W, Vitetta, Ellen S
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.12.1979
Subjects
Animals
Chemical Precipitation
Chromatography, Ion Exchange
Chromatography, Liquid
Genetic Code
H-2 Antigens
Immune Sera - pharmacology
Isoantigens
Major Histocompatibility Complex
Mice
Mice, Inbred A
Peptides
Rabbits
Recombination, Genetic
Trypsin - pharmacology
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Summary:The alpha and beta subunits of the murine I-A alloantigens from several H-2 haplotypes were examined by comparative tryptic peptide mapping by using double label (3H and 14C) techniques. Significant structural variation between alleles was detected in both subunits. Tryptic digests of the alpha polypeptides from s, b, and d showed only 65% co-elution with k; beta-chains from s, b, d, and r were about 50% similar to the k beta subunit. Peptide analysis of the Ak subunits from intra-H-2 recombinant strains indicated that both the alpha and beta polypeptides are encoded within the I-A subregion.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0022-1767
1550-6606