T Cell Recognition in the Mixed Lymphocyte Response: I. Non-T, Radiation-Resistant Splenic Adherent Cells Are the Predominant Stimulators in the Murine Mixed Lymphocyte Reaction

• Published in: The Journal of immunology (1950) Vol. 123; no. 2; pp. 903 - 909
• Main Authors: Ahmann, Gerald B, Nadler, Paul I, Birnkrant, Alan, Hodes, Richard J
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.08.1979
• Subjects: Animals; B-Lymphocytes - immunology; Cell Adhesion; Classification; Epitopes; Histocompatibility Antigens - genetics; Histocompatibility Antigens - immunology; Immune Sera - pharmacology; Lymphocyte Culture Test, Mixed; Lymphocytes; Male; Mice; Mice, Inbred A; Mice, Inbred BALB C; Mice, Inbred C57BL; Rabbits; Spleen - immunology; T-Lymphocytes - immunology
• ISSN: 0022-1767; 1550-6606

The ability of subpopulations of murine spleen cells to stimulate a mixed lymphocyte response (MLR) was studied. It was found that T cells (nylon-nonadherent spleen cells) and B cells [G-10 passed and treated with rabbit anti-mouse brain serum (RAMB) and complement (C)] were poor stimulators of an MLR. In contrast, whole spleen cells or B cells plus adherent cells (RAMB +C-treated spleen cells) produced good stimulation. However, a non-T, radiation-resistant splenic adherent cell (SAC) population was up to 20 to 50 times more efficient as a stimulator of an MLR on a per cell basis than an unseparated spleen population. These SAC were shown to express Ia determinants encoded by genes in I-A and I-E/C. These results suggest that Ia+ SAC may be the predominant stimulating cells in spleen cell populations, and the preferential target for T cell recognition in cell interaction events.