Developmental changes of Ca2+, PO4, and calcitriol metabolism in spontaneously hypertensive rats

• Published in: The American journal of physiology Vol. 259; no. 1 Pt 2; p. F104
• Main Authors: Bourgouin, P, Lucas, P, Roullet, C, Pointillart, A, Thomasset, M, Brami, M, Comte, L, Lacour, B, Garabédian, M, McCarron, D A
• Format: Journal Article
• Language: English
• Published: United States 01.07.1990
• Subjects: Aging - metabolism; Animals; Calcifediol - blood; Calcitriol - blood; Calcitriol - metabolism; Calcium - administration & dosage; Calcium - metabolism; Calcium-Binding Proteins - analysis; Diet; Hypertension - metabolism; Intestines - analysis; Male; Phosphates - metabolism; Rats; Rats, Inbred SHR; Rats, Inbred WKY
• ISSN: 0002-9513

We have measured Ca and P balance, serum calcitriol, and vitamin D-dependent intestinal calcium-binding protein (CaBP9k) in the spontaneously hypertensive rat (SHR) and its normotensive control, the Wistar-Kyoto rat (WKY) at 4-5 and again at 13-14 wk of age. In rats on a 1% Ca diet, P balance was significantly more positive in the 5-wk-old SHR than WKY (P less than 0.01); Ca balance tended to be greater in the 5-wk-old SHR. In contrast, in the 14-wk-old SHR, P and Ca balance were less positive than in the WKY (P was 5.8 +/- 1.3 vs. 13 +/- 1.7 mg/day, P less than 0.01, and Ca was 53 +/- 4.6 vs. 67 +/- 2.7 mg/day, P less than 0.05). On the 1% Ca diet, plasma calcitriol levels of the 5-wk-old SHR were higher than those of the WKY (58 +/- 3.2 vs. 40 +/- 2.1 pg/ml, P less than 0.002), whereas at 12 wk there was no difference. On Ca-deficient (0.1%) diets, plasma calcitriol was increased in 5-wk-old and 12-wk-old SHR and WKY, compared with the 1% Ca diet (P less than 0.001). Calcitriol metabolic clearance rate was the same in the 13-wk-old SHR and WKY on either Ca diet. Intestinal CaBP9k content of the 5-wk-old (38 +/- 2.6 vs. 40 +/- 3.9 micrograms/mg) and the 12-wk-old (11 +/- 1.6 vs. 14 +/- 1.4 micrograms/mg) SHR and WKY were similar on the 1% Ca diet.