The Plasmodium falciparum Translationally Controlled Tumor Protein

Plasmodium falciparum secretes a homologue of the translationally controlled tumor protein (TCTP) into serum of infected individuals, although its role in pathogenesis or virulence is unknown. To determine the effect of P. falciparum TCTP on B cells as compared to human TCTP, fluorescently labeled p...

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Bibliographic Details
Published inPloS one Vol. 9; no. 1; p. e85514
Main Authors Calderón-Pérez, Berenice, Xoconostle-Cázares, Beatriz, Lira-Carmona, Rosalía, Hernández-Rivas, Rosaura, Ortega-López, Jaime, Ruiz-Medrano, Roberto
Format Journal Article
LanguageEnglish
Published Public Library of Science 17.01.2014
Subjects
Analysis
B cells
Malaria
Recombinant proteins
Tumors
Virulence (Microbiology)
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Summary:Plasmodium falciparum secretes a homologue of the translationally controlled tumor protein (TCTP) into serum of infected individuals, although its role in pathogenesis or virulence is unknown. To determine the effect of P. falciparum TCTP on B cells as compared to human TCTP, fluorescently labeled proteins were incubated on primary cultures of mouse splenic B cells and analyzed by flow cytometry and confocal microscopy. Our results indicate that both recombinant proteins are incorporated into B cells, but differ significantly in their rate and percentage of incorporation, being significantly higher for P. falciparum TCTP. Furthermore, P. falciparum TCTP showed a lower B cell proliferative effect than human TCTP, suggesting a mechanism through which the former could interfere in the host's immune response.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0085514