Protein Phosphatase Magnesium Dependent 1A

The serine/threonine phosphatase type 2C (PPM1A) has a broad range of substrates, and its role in regulating stress response is well established. We have investigated the involvement of PPM1A in the survival and differentiation processes of PC6-3 cells, a subclone of the PC12 cell line. This cell li...

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Bibliographic Details
Published inPloS one Vol. 7; no. 2; p. e32438
Main Authors Shohat, Meytal, Ben-Meir, Daniella, Lavi, Sara
Format Journal Article
LanguageEnglish
Published Public Library of Science 27.02.2012
Subjects
Apoptosis
Nerve growth factor
Neurons
Phosphatases
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Summary:The serine/threonine phosphatase type 2C (PPM1A) has a broad range of substrates, and its role in regulating stress response is well established. We have investigated the involvement of PPM1A in the survival and differentiation processes of PC6-3 cells, a subclone of the PC12 cell line. This cell line can differentiate into neuron like cells upon exposure to nerve growth factor (NGF). Overexpression of PPM1A in naive PC6-3 cells caused cell cycle arrest at the G2/M phase followed by apoptosis. Interestingly, PPM1A overexpression did not affect fully differentiated cells. Using PPM1A overexpressing cells and PPM1A knockdown cells, we show that this phosphatase affects NGF signaling in PC6-3 cells and is engaged in neurite outgrowth. In addition, the ablation of PPM1A interferes with NGF-induced growth arrest during differentiation of PC6-3 cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0032438