A MYB transcription factor, BnMYB2, cloned from ramie

• Published in: PloS one Vol. 15; no. 5; p. e0233375
• Main Authors: Zhu, Shoujing, Shi, Wenjuan, Jie, Yucheng, Zhou, Qingming, Song, Chenbo
• Format: Journal Article
• Language: English
• Published: Public Library of Science 18.05.2020
• Subjects: Analysis; Arabidopsis; Arabidopsis thaliana; Cadmium; Cloning; DNA; DNA binding proteins; Ecological stress; Environmental aspects; Fluorescence; Genes; Genetic aspects; Identity; Novels; Physiological aspects; Protein binding; Proteins; Ramie; Time; Transcription factors; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0233375

MYB-related transcription factors play important roles in plant development and response to various environmental stresses. In the present study, a novel MYB gene, designated as BnMYB2 (GenBank accession number: MF741319.1), was isolated from Boehmeria nivea using rapid amplification of cDNA ends (RACE) and RT-PCR on a sequence fragment from a ramie transcriptome. BnMYB2 has a 945 bp open reading frame encoding a 314 amino acid protein that contains a DNA-binding domain and shares high sequence identity with MYB proteins from other plant species. The BnMYB2 promoter contains several putative cis-acting elements involved in stress or phytohormone responses. A translational fusion of BnMYB2 with enhanced green fluorescent protein (eGFP) showed nuclear and cytosolic subcellular localization. Real-time PCR results indicated that BnMYB2 expression was induced by Cadmium (Cd) stress. Overexpression of BnMYB2 in Arabidopsis thaliana resulted in a significant increase of Cd tolerance and accumulation. Thus, BnMYB2 positively regulated Cd tolerance and accumulation in Arabidopsis, and could be used to enhance the efficiency of Cd removal with plants.