Association Of GSTMI, GSTTI And GSTPI Polymorphisms With Breast Cancer Among Jordanian Women

• Published in: OncoTargets and therapy p. 7757
• Main Authors: Al-Eitan, Laith N, Rababa'H, Doaa M, Alghamdi, Mansour A, Khasawneh, Rame H
• Format: Journal Article
• Language: English
• Published: Dove Medical Press Limited 01.09.2019
• Subjects: Breast cancer; Cancer research; Electrophoresis; Epidemiology; Genes; Genetic aspects; Genetic polymorphisms; Genetic research; Glutathione transferase; Medical research; Risk factors; Tumors; Canada
• ISSN: 1178-6930; 1178-6930
• DOI: 10.2147/OTT.S207255

Purpose: Genetic predisposition to disease has become one of the most investigated risk factors in recent years, and breast cancer (BC) is no exception. In this study, we investigated specific genetic variants of three candidate genes belonging to the glutathione-S-transferase superfamily that have been implicated in increased risk of cancers. Materials and methods: This case-control study comprised 241 Jordanian women who were diagnosed with BC in addition to 219 matched controls. Gel electrophoresis of PCR products was used to visualize and genotype both the GSTM1 and GSTT1 genes, while PCR-RFLP was employed to genotype the rs1695 of the GSTP1 gene. Results: Our findings did not reveal any correlation between the investigated polymorphisms of GST genes and BC risk among Jordanian women. Otherwise, the combination of GSTM1 entire gene deletion and (GG) genotype of GSTP1 polymorphism (rs1695) was significantly associated with BC with p-value <0.05 (i.e. p-value was not significant after correcting for multiple comparison). Conclusion: We suggest that the interaction between GSTM1 polymorphism and rs1695 of GSTP1 may influence BC development and progression among Jordanian women. More epidemiological studies are needed to provide a baseline for the underlying role of GSTs polymorphisms in tumorigenesis. Keywords: breast, cancer, genetic variation, GST genes, polymorphisms