T cell receptor-V[beta] repertoires in lung and blood CD4+ and CD8+T cells of pulmonary sarcoidosis patients

Background Sarcoidosis patients have accumulations of activated CD4.sup.+ T cells in affected organs, such as the lungs. T cell receptor (TCR) V[beta]-chain usage has been incompletely characterized in these patients. Methods We surveyed the TCR V[beta] usage in CD4.sup.+ and CD8.sup.+ T cells in br...

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Published inBMC pulmonary medicine Vol. 14
Main Authors Ahlgren, Kerstin M, Ruckdeschel, Tina, Eklund, Anders, Wahlström, Jan, Grunewald, Johan
Format Journal Article
LanguageEnglish
Published London BioMed Central Ltd 22.03.2014
BioMed Central
Subjects
Analysis
Antibodies
Blood
Disease
Genes
Hospitals
Immune system
Lungs
Lymphocytes
Medicine
Pulmonology
Sarcoidosis
Studies
Surveys
T cell receptors
Viral antibodies
Sweden
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Summary:Background Sarcoidosis patients have accumulations of activated CD4.sup.+ T cells in affected organs, such as the lungs. T cell receptor (TCR) V[beta]-chain usage has been incompletely characterized in these patients. Methods We surveyed the TCR V[beta] usage in CD4.sup.+ and CD8.sup.+ T cells in bronchoalveolar lavage (BAL) cells and peripheral blood mononuclear cells (PBMC) from 15 HLA-typed Scandinavian sarcoidosis patients. In addition, PBMC from 9 healthy volunteers and BAL cells from three of them were examined. Using 21 V[beta] family-specific antibodies, we covered approximately 70% of all V[beta] chains. Results In BAL T cells from sarcoidosis patients, we identified 16 CD4.sup.+ T cell expansions in 271 analyses (5.9%) and 21 CD8.sup.+ expansions in 240 analyses (8.7%). In PBMC we found 9 CD4.sup.+ expansions in 276 analyses (3.3%) and 12 CD8.sup.+ expansions out of 263 analyses (4.6%). Consistent with previous studies we found V[beta]8 and V[beta]16 expansions in sarcoidosis patients' lungs. In addition, we found lung restricted V[beta]22 expansions in three HLA DRB1 03.sup.+ patients. However, we found no statistically significant difference in frequency of expansions between patients and healthy controls. Conclusions The identified T cell expansions in present study indicate specific antigen recognition in the lungs of sarcoidosis patients. Keywords: T cell, T cell receptor, Sarcoidosis, Bronchoalveolar Lavage, V[beta]-repertoire, flow cytometry
ISSN:1471-2466
1471-2466
DOI:10.1186/1471-2466-14-50