Rapid and Simultaneous Extraction of Bisabolol and Flavonoids from IGymnosperma glutinosum/I and Their Potential Use as Cosmetic Ingredients

• Published in: Separations Vol. 10; no. 7
• Main Authors: Gómez-Patiño, Mayra Beatriz, Leyva Pérez, Juan Pablo, Alcibar Muñoz, Marcia Marisol, Arzate-Vázquez, Israel, Arrieta-Baez, Daniel
• Format: Journal Article
• Language: English
• Published: MDPI AG 01.07.2023
• Subjects: Fatty acids; Health aspects; Isoflavones; Mexico
• ISSN: 2297-8739; 2297-8739
• DOI: 10.3390/separations10070406

Gymnosperma glutinosum is a plant popularly known as “popote”, “tatalencho”, “tezozotla” or “pegajosa”, and it is used in traditional medicine in the region of Tehuacán, Puebla (Mexico), for the treatment of jiotes and acne and to cure diarrhea using the aerial parts in infusions. To analyze the phytochemical composition, we have developed a rapid protocol for the extraction and separation of the components of the aerial parts of G. glutinosum. After a maceration process, chloroformic and methanolic extracts were obtained and analyzed. Extracts were evaluated by GC-MS (gas chromatography-mass spectrometry), and their composition revealed the presence of (−)-α-bisabolol (BIS) as the main component in the chloroformic extract, which was isolated and analyzed by [sup.1]H NMR to confirm its presence in the plant. The analysis of methanolic extracts by UPLC-MS (ultra-performance liquid chromatography-mass spectrometry) revealed the occurrence of six methoxylated flavones with m/z 405.08 (C[sub.19]H[sub.18]O[sub.10]), m/z 419.09 (C[sub.20]H[sub.20]O[sub.10]) and m/z 433.11 (C[sub.21]H[sub.22]O[sub.10]), and a group of C20-, C18-hydroxy-fatty acids, which give the plant its sticky characteristic. The presence of BIS, an important sesquiterpene with therapeutic skin effects, as well as some antioxidant compounds such as methoxylated flavones and their oils, could play an important role in cosmetology and dermatology formulations.