Complete Chloroplast Genome of IKrascheninnikovia ewersmanniana/I: Comparative and Phylogenetic Analysis

• Published in: Genes Vol. 15; no. 5
• Main Authors: Wei, Peng, Li, Youzheng, Ke, Mei, Hou, Yurong, Aikebaier, Abudureyimu, Wu, Zinian
• Format: Journal Article
• Language: English
• Published: MDPI AG 01.05.2024
• Subjects: Deserts; Genomes; Genomics; Grasslands; Phylogeny; China
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes15050546

Krascheninnikovia ewersmanniana is a dominant desert shrub in Xinjiang, China, with high economic and ecological value. However, molecular systematics research on K. ewersmanniana is lacking. To resolve the genetic composition of K. ewersmanniana within Amaranthaceae and its systematic relationship with related genera, we used a second-generation Illumina sequencing system to detect the chloroplast genome of K. ewersmanniana and analyze its assembly, annotation, and phylogenetics. Total length of the chloroplast genome of K. ewersmanniana reached 152,287 bp, with 84 protein-coding genes, 36 tRNAs, and eight rRNAs. Codon usage analysis showed the majority of codons ending with base A/U. Mononucleotide repeats were the most common (85.42%) of the four identified simple sequence repeats. A comparison with chloroplast genomes of six other Amaranthaceae species indicated contraction and expansion of the inverted repeat boundary region in K. ewersmanniana, with some genes (rps19, ndhF, ycf1) differing in length and distribution. Among the seven species, the variation in non-coding regions was greater. Phylogenetic analysis revealed Krascheninnikovia ceratoides, Dysphania ambrosioides, Dysphania pumilio, and Dysphania botrys to have a close monophyletic relationship. By sequencing the K. ewersmanniana chloroplast genome, this research resolves the relatedness among 35 Amaranthaceae species, providing molecular insights for germplasm utilization, and theoretical support for studying evolutionary relationships.