Determination of Cytotoxic and Anticandidal Activities of Three Verbascum L. Species from Turkey: V. cheiranthifolium Boiss. var. asperulum Murb. Monorg., Verbascum pynostachyum Boiss. Heldr ve Verbascum orgyale Boiss. Heldr

Purpose of this study is to determine of cytotoxic and anticandidal activities of Verbascum cheiranthifolium Boiss. var. asperulum (Boiss.) Murb. Monorg., Verbascum pynostachyum Boiss. & Heldr and Verbascum orgyale Boiss. & Heldr belonging to Verbascum genus growing in Turkey. The cytotoxic...

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Bibliographic Details
Published inTurkish Journal of Pharmaceutical Sciences Vol. 13; no. 3; p. 318
Main Authors Kucuk, Sevim, Ozdemir, Filiz, Iscan, Gokalp, Incesu, Zerrin
Format Journal Article
LanguageEnglish
Published Galenos Yayinevi Tic. Ltd 01.12.2016
Subjects
Antifungal agents
Candidiasis
Care and treatment
Chemical properties
Composition
Health aspects
Materia medica, Vegetable
Methanol
Mullein
Pharmaceutical research
Plant extracts
Turkey
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Summary:Purpose of this study is to determine of cytotoxic and anticandidal activities of Verbascum cheiranthifolium Boiss. var. asperulum (Boiss.) Murb. Monorg., Verbascum pynostachyum Boiss. & Heldr and Verbascum orgyale Boiss. & Heldr belonging to Verbascum genus growing in Turkey. The cytotoxic effects of methanolic extract of Verbascum cheiranthifolium var. asperulum, V. pycnostachyum and V. orgyale species on the cervical (HeLa) and ovarian cancer (Skov-3) cells were investigated using colorimetric assay. The results indicated that methanolic-extract of V. pycnostachyum had a promising toxic effect on both cell lines as compared to the other species. Furthermore, this effect was more significant on Skov-3 cells rather than HeLa cells. Anticandidal effects of the methanolic extracts were evaluated in comparison with standard antifungal agents according to Clinical Laboratory Standards Institute (CLSI) reference methods, for the first time here. V. pynostactum and V. orygale extracts were demonstrated stronger inhibitory effects than the V. cheriantifolium var. asperulum. Remarkably, Candida krusei was inhibited by V. pycnostachyum extract at the concentration of the 62.5 [micro]g/mL. Key words: Scrophulariaceae, Verbascum, Cytotoxicity, Anticandidal activities Bu calismada Turkiye'de yetisen Verbascum L. cinsine ait uc turun; Verbascum cheiranthifolium Boiss. var. asperulum (Boiss.) Murb. Monorg., V. pycnostachyum Boiss. & Heldr, ve V. orgyale Boiss. & Heldr. turlerinin sitotoksik ve antikandidal aktivitelerinin belirlenmesi amaclanmistir. Verbascum cheiranthifolium var. asperulum, V. pycnostachyum and V. orgyale turlerinin metanol ekstrelerinin sitotoksik etkileri servikal (HeLa) ve ovaryum kanser (Skov-3) hucrelerinde kolorimetrik metod kullanilarak arastirilmistir. Elde edilen sonuclar; V. pycnostachyum turunun metanol ekstresinin diger turlere oranla her iki hucre hattinda da umut verici toksik etkiye sahip oldugu gosterilmistir. Buna ek olarak; bu etki Skov-3 hucrelerinde HeLa hucrelerine kiyasla daha anlamlidir. Uc ture ait metanol ekstresinin antikandidal etkileri "Klinik Laboratuvar Standartlar Enstitusu" (CLSI)'nun mikrodilusyon standart protokolleri kullanilarak standart antifungal ajanlarla karlilastirmali sekilde ilk kez bu calisma ile ortaya konmustur. V. pycnostachyum ve V. orygale ekstreleri V. cheriantifolium'a gore daha kuvvetli inhibitor etkiler gostermistir. Verbascum pycnostachyum ekstresi dikkat cekici olarak Candida krusei'yi, 62.5 g/mL konsantrasyonda inhibe etmistir. Anahtar kelimeler: Scrophulariaceae, Verbascum, Sitotoksisite, Antikandidal aktivite
ISSN:1304-530X
DOI:10.4274/tjps.2016.06