Antimicrobial susceptibility profiles of Staphylococcus spp. from domestic and wild animals/ Perfil de suscetibilidade antimicrobiana e diversidade de Staphylococcus spp. de animais domesticos e silvestres

The aim of this study was to determine the prevalence and diversity of veterinary clinical isolates of Staphylococcus and analyze their antimicrobial susceptibility. One hundred Staphylococcus spp. clinical isolates from domestic and wild animals were subjected to partial sequencing of the 16S rRNA...

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Published inCiência rural Vol. 46; no. 12; p. 2148
Main Authors de Godoy, Isabela, Moraes, Danny Franciele da Silva Dias, Pitchenin, Leticia Camara, Rosa, Janaina Marcela Assuncao, Kagueyama, Francielle Cristina, da Silva, Aline de Jesus, Dutra, Valeria, Nakazato, Luciano
Format Journal Article
LanguageEnglish
Published Universidade Federal de Santa Maria 01.12.2016
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Summary:The aim of this study was to determine the prevalence and diversity of veterinary clinical isolates of Staphylococcus and analyze their antimicrobial susceptibility. One hundred Staphylococcus spp. clinical isolates from domestic and wild animals were subjected to partial sequencing of the 16S rRNA gene to species determination. Antimicrobial susceptibility was obtained by a disk diffusion test against six antibiotics: amoxicillin (AMX), cephalexin (LEX), ciprofloxacin (CIP), erythromycin (ERY), gentamicin (GEN) and trimethoprim-sulfamethoxazole (SXT). The most common specie was S. pseudintermedius (61%, 61/100) and resistance to ERY (57%, 57/100), SXT (50%, 50/100) and AMX (46%, 46/100) was detected most frequently In total, 40% (40/100) of Staphylococcus spp. exhibited a multidrug-resistant (MDR) phenotype. Results of this study emphasize that animals are reservoir of MDR Staphylococcus spp. Key words: Staphylococcus spp., antimicrobial resistance, multidrug resistance. O objetivo do presente trabalho foi determinar a prevalencia e diversidade de isolados clinicos veterinarios de Staphylococcus e analisar o perfil de suscetibilidade a antimicrobianos. Um total de 100 Staphylococcus spp. isolados de amostras clinicas de animais domesticos e silvestres foram submetidos ao sequenciamento parcial do gene 16S rRNA, para determinacao da especie. A suscetibilidade antimicrobiana foi obtida por meio da tecnica de Disco Difusao contra seis antibioticos: amoxicilina, cefalexina, ciprofloxacina, eritromicina, gentamicina e sulfazotrim. A especie mais frequente foi S. pseudintermedius (61%, 61/100) e a resistencia a eritromicina (57%, 57/100), Sulfazotrim (50%, 50/100) e Amoxicilina (46%, 46/100) foi detectada mais frequentemente. No total, 40% (40/100) dos Staphylococcus spp. demonstraram um fenotipo de multirresistencia a drogas (MRD). Os resultados obtidos neste trabalho reforcam o fato de que animais sao reservatorios de Staphylococcus spp. MRD. Palavras-chave: Staphylococcus spp., resistencia a antimicrobianos, multirresistencia a drogas.
ISSN:0103-8478
1678-4596
DOI:10.1590/0103-8478cr20160373