EFFECTS OF ERYTHROPOIETIN PRETREATMENT ON LIVER, KIDNEY, HEART TISSUE IN PENTYLENTETRAZOL-INDUCED SEIZURES; EVALUATION IN TERMS OF OXIDATIVE MARKERS, PROLIDASE AND SIALIC ACID/PENTILENTETRAZOL-INDUKLU NOBETLERDE ERITROPOIETIN ON TEDAVISININ KARACIGER, BOBREK, KALP DOKUSU UZERINE ETKILERI; OKSIDATIF MARKIRLAR, PROLIDAZ VE SIALIK ASIT ACISINDAN DEGERLENDIRME

• Published in: İstanbul Tıp Fakültesi dergisi = Journal of the Istanbul Faculty of Medicine Vol. 84; no. 4; p. 464
• Main Authors: Kapucu, Aysegul, Kaptan, Zulal, Dar, Kadriye Akgun, Kaleler, Islim, Uzum, Gulay
• Format: Journal Article
• Language: English
• Published: AVES 01.12.2021
• Subjects: Care and treatment; Complications and side effects; Erythropoietin; Health aspects; Heart; Kidneys; Liver; Oxidative stress; Seizures (Medicine); Turkey
• ISSN: 1305-6433
• DOI: 10.26650/IUITFD.2021.883402

Objective: The effects of erythropoietin (EPO) which has been frequently studied as an anti-epileptic agent, on peripheral tissues have not been investigated. This study investigated the effects on malondialdehyde (MDA), advanced protein oxidation products (AOPP), superoxide dismutase (SOD), prolidase and sialic acid (SA) levels in the heart, kidney and liver tissues of EPO pretreatment in pentylenetetrazole (PTZ)-induced seizures. Material and Method: Thirty three male adult rats were divided into three groups. A saline-injected control group, a 60 mg/kg PTZ-injected group to induce seizures and a 3000 IU/kg EPO-injected group 24 hours before seizures. After seizure severity and seizure latency were scored, the rats sacrificed, the tissues were immediately removed for biochemical analyses. Results: The PTZ-induced seizures increased MDA in kidney (p<0.01) and AOPP in liver (p<0.05) but didn't alter these markers in heart tissue. In all three tissues, SOD didn't change due to seizures. The SA levels increased in the heart (p<0.001), decreased in the kidney (p<0.001), and were unchanged in liver. Prolidase increased (p<0.05) only in kidney, and was unchanged in other tissues. EPO-pretreatment decreased seizure severity and increased seizure latency. It prevented the increase in MDA in the kidney (p<0.01) but increased AOPP (p<0.05) and decreased SOD (p<0.01) and further increased prolidase more than the seizures increased (p<0.01). EPO-pretreatment prevented the increase in AOPP in the liver (p<0.05) but was ineffective in PTZ-induced SA changes in the heart and kidney. Conclusion: We think that the increase in the heart SA level in seizures is an original finding and deserves investigation in the context of seizure-related cardiac arrhytmias. Also, despite the EPO's anti-seizure effect, increased protein oxidaiton and prolidase, especially in the kidney, is an other important finding that needs further research. Keywords: Erythropoietin, PTZ-induced seizures, oxidative stress markers, sialic acid, prolidase, peripheral tissues Amac: Antiepileptik ajan olarak siklikla calisilan eritropoietinin (EPO)'nun periferik dokular uzerindeki etkileri arastirilmamistir. Bu calismada pentilentetrazol (PTZ) ile induklenen nobetlerde EPO on tedavisinin kalp, bobrek ve karaciger dokularinda malondialdehit (MDA), ileri protein oksidasyon urunleri (AOPP), superoksid dismutaz (SOD), prolidaz ve sialik asit (SA) seviyelerine etkisi arastirildi. Gerec ve Yontem: Otuz uc eriskin erkek sican uc gruba ayrildi. Salin enjekte edilmis kontrol grubu, nobetleri induklemek icin 60 mg/kg PTZ enjekte edilmis grup, nobetlerden 24 saat once 3000 IU/kg EPO enjekte edilmis grup. Nobet siddeti ve nobet gecikmesi puanlandiktan sonra, sicanlar sakrifiye edildi, dokular biyokimyasal analizler icin hemen cikarildi. Bulgular: Pentilentetrazol ile induklenen nobetler, bobrekte MDA (p<0,01) ve karacigerde AOPP'yi arttirdi (p<0,05), ancak kalp dokusunda bu markirlari degistirmedi. Her uc dokuda da SOD nobetler nedeniyle degismedi. Kalpte SA artti (p<0,001), bobrekte azaldi (p<0,001), karacigerde degismedi. Prolidaz sadece bobrekte artti (p<0,05), diger dokularda degismedi. EPO on tedavisi nobet siddetini azaltti ve nobet latansini artirdi. EPO bobrekte MDA artisini engelledi (p<0,01), ancak AOPP'yi artirdi (p<0,05) ve SOD'u azaltti (p<0,01) ve prolidazi nobetlerin arttirdi gindan daha fazla artirdi (p<0,01). EPO on tedavisi, karacigerde AOPP artisini onledi (p<0,05), ancak kalp ve bobrekte PTZ'nin neden oldugu SA degisikliklerinde etkisizdi. Sonuc: Nobetlerde kalp dokusundaki SA artisinin nobet-iliskili kardiak aritmiler baglaminda arastirmayi hak eden orijinal bulgu oldugunu dusunuyoruz. Ayrica EPO on tedavisinin nobet engelleyici etkisine ragmen ozellikle bobrek dokusunda artmis protein oksidasyonu ve prolidaz da ileri arastirmayi gerektiren diger onemli bulgudur. Anahtar Kelimeler: Eritropoietin, PTZ-induklu nobetler, oksidatif stres belirtecleri, sialik asit, prolidaz, periferik dokular