Pharmacodynamic study of [sup.131]I-labeled CA215 antibody on an animal model of estrogen-resistant OC-3-VGH ovarian cancer

• Published in: Experimental and therapeutic medicine p. 572
• Main Authors: Liu, Xiang-Yun, Su, Xin, Xie, Chen-Jing, Li, Lei, Yan, Jian-Yan, Sun, Zu-Yue
• Format: Journal Article
• Language: English
• Published: Spandidos Publications 01.02.2015
• Subjects: Dosage and administration; Drug therapy; Ovarian cancer; Patient outcomes; Pharmacology, Experimental; Tumor antigens
• ISSN: 1792-0981
• DOI: 10.3892/etm.2015.2517

The aim of the present study was to explore the inhibitory effect of [sup.131]I-labeled ovarian cancer antigen 215 ([sup.131]I-CA215) antibody on human OC-3-VGH ovarian cancer. A subcutaneous transplanted tumor model of estrogen-resistant human OC-3-VGH ovarian cancer in nude mice was established. The model mice were randomly divided into seven groups, which were the negative control (NC), positive control (PC; 60 mg/kg cyclophosphamide), high-dose CA215 antibody (HA; 10 mg/kg), low-dose CA215 antibody (LA; 2 mg/kg), high-dose [sup.131]I-CA215 antibody ([sup.131]I-HA; 10 mg/kg + 125 μCi), medium-dose [sup.131]I-CA215 antibody ([sup.131]I-MA; 6 mg/kg + 75 μCi) and low-dose [sup.131]I-CA215 antibody ([sup.131]I-LA; 2 mg/kg + 25 μCi) groups. Each group received intraperitoneal administration for 14 consecutive days. At 24 h after the final administration, the tumor was removed and weighed to calculate the tumor inhibition rate (TIR) and the relative tumor increase rate (T/C). Compared with the NC group, the HA group, as well as the [sup.31]I-HA and [sup.131]I-MA antibody groups, exhibited significantly inhibited tumor growth. The relative T/C values were 54, 30 and 48%, respectively, and the TIRs were 33.59, 64.89 and 45.80%, respectively. All differences were statistically significant. The difference between the HA and [sup.131]I-HA groups also presented statistical significance. CA215 and [sup.131]I-CA215 antibodies can markedly inhibit OC-3-VGH ovarian cancer. The high-dose [sup.131]I-CA215 antibody demonstrated a clear synergetic effect. Key words: [sup.131]I-CA215 antibody, estrogen-resistant, ovarian cancer, animal model