Simultaneous biosensing of biogenic amines: signal analysis and biosensor calibration/Biogeensete amiinide heaegne m ramine biosensoriga: biosensori signaali anal s ja selle kalibreerimine

• Published in: Proceedings of the Estonian Academy of Sciences Vol. 73; no. 3; p. 203
• Main Authors: Kivirand, Kairi, Rinken, Priit, Rinken, Toonika
• Format: Journal Article
• Language: English
• Published: Estonian Academy Publishers 01.09.2024
• Subjects: Analysis; Biodegradation; Detectors; Histamine; Medical equipment; Physiological apparatus; Polyamines; Proteins
• ISSN: 1736-6046
• DOI: 10.3176/proc.2024.3.04

The application of biosensors in complex real-world samples is often complicated due to the combined effects and interferences of various compounds on the biosensor signal. However, thorough modelling and chemometric methods allow us to evaluate the impact of different analytes and calibrate these biosensors for the multiplexed analyses of the targeted compounds. A typical multianalyte mixture includes biogenic amines, produced during the putrefaction of proteins. Meanwhile, the detection of particular biogenic amines is a valuable tool for assessing the freshness and quality of a wide variety of protein-containing foods. In the current study, we analysed the signal of biosensors for the simultaneous detection of four major biogenic amines (cadaverine, putrescine, histamine, and spermidine) and proposed two different approaches for their multivariate calibration. The evaluation of the proposed models and the calculation of their characteristic coefficients were based on experimental data from over three hundred different mixtures with randomly varying substrate concentrations. Keywords: biogenic amines, detection, biosensor, multiplex analysis, chemometrics. Biosensorite praktiline kasutamine reaalsetes proovides, kus leidub palju m detavat signaali m jutavaid komponente, on tihti keerukas komponentide vastastikuse m ju t ttu. Siiski v imaldab signaali modelleerimine ja kemomeetriliste meetodite kasutamine biosensorite kalibreerimist mitme anal di heaegseks m ramiseks biosensorite v ljundsignaali alusel. ks olulisemaid probleemseid proove, kus on vaja igap evaselt m rata korraga mitme komponendi sisaldust, on biogeensete amiinide segud, mis tekivad valkude lagunemisel ja kus biogeensete amiinide kontsentratsioon on hea indikaator valku sisaldavate toodete (nt kalatooted, liha, juust, vein) kvaliteedi hindamisel. K esolevas t s uuritigi v imalusi diamiini oks daasil (EC 1.4.3.22) p hineva biosensori v ljundsignaali modelleerimiseks ning selle alusel sensori kalibreerimiseks nelja olulisema biogeense amiini (kadaveriin, putrestsiin, histamiin ja spermidiin) heaegseks m ramiseks nende segudes. Signaali modelleerimiseks pakuti v lja kaks erinevat l henemist: esimene p hines toimuvate f sikalis-keemiliste protsesside arvestamisel ja teine m ratavate biogeensete amiinide kontsentratsioonide erineval formaalloogilisel kombineerimisel. V ljapakutud mudelite korreleerimisel rohkem kui kolmesajal m tmisel saadud eksperimentaalsete andmetega leitud korrelatsioonikoefitsientide v rtus oli vahemikus 0,54 kuni 0,85. Selgus, et f sikalis-keemilise mudeli korral, mis v ttis arvesse substraatide difusiooni, kasutatud ens mi aktiivsust erinevate substraatide suhtes ja spermidiini k rgemate kontsentratsioonide inhibeerivat m ju ens mile, olid eksperimentaalsete tulemuste h lbed erinevalt v ljapakutud formaalsest mudelist mittes stemaatilised. Saadud tulemuste alusel v ib j reldada, et biosensorite kalibreerimiseks on eelistatud f sikalis-keemiline mudel. V ljapakutud l henemine on perspektiivne ka teiste biosensorite kalibreerimiseks, mida kasutatakse mitme anal di m ramiseks nende segudes.