Identification of ICNGCs/I in IGlycine max/I and Screening of Related Resistance Genes after IFusarium solani/I Infection

• Published in: Biology (Basel, Switzerland) Vol. 12; no. 3
• Main Authors: Cui, Yuxing, Wang, Jingxuan, Bai, Yingxue, Ban, Liping, Ren, Junda, Shang, Qiaoxia, Li, Weiyu
• Format: Journal Article
• Language: English
• Published: MDPI AG 01.03.2023
• Subjects: Amino acids; Analysis; Arabidopsis thaliana; Cellular signal transduction; Developmental biology; Drug resistance in microorganisms; Genes; Health aspects; Infection; Physiological aspects; Soybean; China
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology12030439

Glycine max diseases have always been an issue in China, with root rot caused by Fusarium solani being the most serious issue in this regard. Here, the mechanism of action of genes showing F. solani resistance was studied by analysing the differential expression of the transcriptome of this plant after pathogen infection to provide a theoretical reference for the breeding of disease-resistant soybeans. Thus, four genes that regulate the concentration of inorganic ions inside and outside the membrane via the transmembrane ion channel and participate in stress regulation via signal transduction were identified. Cyclic nucleotide-gated channels (CNGCs), non-selective cation channels localised on the plasmalemma, are involved in growth, development, and regulatory mechanisms in plants during adverse stress. To date, CNGC gene families in multiple crops have been identified and analysed. However, there have been no systematic studies on the evolution and development of CNGC gene families in legumes. Therefore, in the present study, via transcriptome analysis, we identified 143 CNGC genes in legumes, and thereafter, classified and named them according to the grouping method used for Arabidopsis thaliana. Functional verification for disease stress showed that four GmCNGCs were specifically expressed in the plasmalemma during the stress process. Further, functional enrichment analysis showed that their mode of participation and coordination included inorganic ion concentration regulation inside and outside the membrane via the transmembrane ion channel and participation in stress regulation via signal transduction. The CNGC family genes in G. max involved in disease stress were also identified and physiological stress response and omics analyses were also performed. Our preliminary results revealed the basic laws governing the involvement of CNGCs in disease resistance in G. max, providing important gene resources and a theoretical reference for the breeding of resistant soybean.