Structure and Properties of Exopolysaccharide Produced by IGluconobacter frateurii/I and Its Potential Applications

• Published in: Polymers Vol. 16; no. 7
• Main Authors: Ning, Yingying, Cao, Huiying, Zhao, Shouqi, Gao, Dongni, Zhao, Dan
• Format: Journal Article
• Language: English
• Published: MDPI AG 01.04.2024
• Subjects: Gram-negative bacteria; Identification and classification; Polysaccharides; Properties; Structure; China
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym16071004

An exopolysaccharide (EPS)-producing bacterium was isolated from apricot fermentation broth and identified as Gluconobacter frateurii HDC-08 (accession number: OK036475.1). HDC-08 EPS is a linear homopolysaccharide mainly composed of glucose linked by α-(1,6) glucoside bonds. It contains C, H, N and S elements, with a molecular weight of 4.774 × 10[sup.6] Da. Microscopically, it has a smooth, glossy and compact sheet structure. It is an amorphous noncrystalline substance with irregular coils. Moreover, the EPS showed surface hydrophobicity and high thermal stability with a degradation temperature of 250.76 °C. In addition, it had strong antioxidant properties against DPPH radicals, ABPS radicals, hydroxyl radicals and H[sub.2] O[sub.2] . The EPS exhibited high metal-chelating activity and strong emulsifying ability for soybean oil, petroleum ether and diesel oil. The milk solidification test indicated that the EPS had good potential in fermented dairy products. In general, all the results demonstrate that HDC-08 EPS has promise for commercial applications as a food additive and antioxidant.