Diagnostic methods for identification of root-knot nematodes species from Brazil/Metodos diagnosticos usados na identificacao de especies do nematoide das galhas do Brasil

• Published in: Ciência rural Vol. 48; no. 2
• Main Authors: da Cunha, Tiago Garcia, Visotto, Liliane Evangelista, Lopes, Everaldo Antonio, Oliveira, Claudio Marcelo Goncalves, God, Pedro Ivo Vieira Good
• Format: Journal Article
• Language: English
• Published: Universidade Federal de Santa Maria 01.02.2018
• Subjects: Brasil
• ISSN: 0103-8478; 1678-4596
• DOI: 10.1590/0103-8478cr20170449

The accurate identification of root-knot nematode (RKN) species (Meloidogyne spp.) is essential for implementing management strategies. Methods based on the morphology of adults, isozymes phenotypes and DNA analysis can be used for the diagnosis of RKN. Traditionally, RKN species are identified by the analysis of the perineal patterns and esterase phenotypes. For both procedures, mature females are required. Over the last few decades, accurate and rapid molecular techniques have been validated for RKN diagnosis, including eggs, juveniles and adults as DNA sources. Here, we emphasized the methods used for diagnosis of RKN, including emerging molecular techniques, focusing on the major species reported in Brazil. Key words: DNA, esterase, Meloidogyne, molecular biology, morphological pattern. A identificacao acurada de especies do nematoide das galhas (NG) (Meloidogyne spp.) e essencial para a implementacao de estrategias de manejo. Metodos baseados na morfologia de adultos, fenotipos de isoenzimas e analise de DNA podem ser usados para a diagnose do NG. Tradicionalmente, as especies de NG sao identificadas pela analise do padrao perineal e fenotipos de esterase. Em ambos os procedimentos, femeas maduras sao necessarias. Nas ultimas decadas, tecnicas moleculares acuradas e rapidas tem sido validadas para a diagnose de NG, incluindo ovos, juvenis e adultos como fontes de DNA. Aqui, nos destacamos os metodos usados para a diagnose de NG, incluindo tecnicas moleculares emergentes, focando nas principais especies encontradas no Brasil. Palavras-chave: DNA, esterase, Meloidogyne, biologia molecular, padrao morfologico.