Colonization and Healthcare-Associated Infection of Carbapenem-Resistant IEnterobacteriaceae/I, Data from Polish Hospital with High Incidence of Carbapenem-Resistant IEnterobacteriaceae,/I Does Active Target Screening Matter?

• Published in: Microorganisms (Basel) Vol. 11; no. 2
• Main Authors: Pawłowska, Iwona, Ziółkowski, Grzegorz, Jachowicz-Matczak, Estera, Stasiowski, Michał, Gajda, Mateusz, Wójkowska-Mach, Jadwiga
• Format: Journal Article
• Language: English
• Published: MDPI AG 01.02.2023
• Subjects: Causes of; Cross infection; Drug resistance in microorganisms; Enterobacter; Enterobacteriaceae; Enterobacteriaceae infections; Genetic aspects; Identification and classification; Nosocomial infections; Risk factors; Statistics; Poland
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11020437

The objective of the study was to analyse the incidence of carbapenem-resistant Enterobacteriaceae (CRE) at a provincial hospital from 2019–2021. Multiplex PCR was used to detect the presence of carbapenemase genes. There were 399 cases of CRE detected in total in the analysed period, including 104 healthcare-associated infections. Out of the isolated CRE, 97.7% were Klebsiella pneumoniae with OXA-48 or KPC genes. Overall, among the identified CRE genes, the most frequently present genes were the ones mediating oxacillinase OXA-48 (71%) and KPC (26%), and significantly less often New Delhi NDM metallo-β-lactamase (2.5%). Moreover, two isolates produced two carbapenemases, i.e., OXA-48 and KPC. The conducted research demonstrates that there is a constant need for continuous monitoring of the occurrence of CRE strains and the hospital antibiotic policy, as well as the implementation of procedures to prevent CRE transmission by medical personnel and hospital support staff.