Mineralization Reduces the Toxicity and Improves Stability and Protective Immune Response Induced by IToxoplasma gondii/I

Vaccination is an ideal strategy for the control and prevention of toxoplasmosis. However, the thermostability and effectiveness of vaccines limit their application. Here, calcium mineralization was used to fabricate Toxoplasma gondii tachyzoites as immunogenic core–shell particles with improved imm...

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Bibliographic Details
Published inVaccines (Basel) Vol. 12; no. 1
Main Authors Li, Ling, Guan, Yong-Chao, Bai, Shao-Yuan, Jin, Qi-Wang, Tao, Jian-Ping, Zhu, Guo-Ding, Huang, Si-Yang
Format Journal Article
LanguageEnglish
Published MDPI AG 01.12.2023
Subjects
Analysis
Biomineralization
Immune response
Methods
Toxoplasma
Vaccination
China
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Summary:Vaccination is an ideal strategy for the control and prevention of toxoplasmosis. However, the thermostability and effectiveness of vaccines limit their application. Here, calcium mineralization was used to fabricate Toxoplasma gondii tachyzoites as immunogenic core–shell particles with improved immune response and thermostability. In the current study, T. gondii RH particles coated with mineralized shells were fabricated by calcium mineralization. The mineralized shells could maintain the T. gondii tachyzoites structural integrity for at least 12 months and weaken the virulence. Immunization of mice with mineralized tachyzoites induced high levels of T. gondii-specific antibodies and cytokines. The immunized mice were protected with a 100% survival rate in acute and chronic infection, and brain cyst burdens were significantly reduced. This study reported for the first time the strategy of calcium mineralization on T. gondii and proved that mineralized tachyzoites could play an immune protective role, thus expanding the application of biomineralization in T. gondii vaccine delivery.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines12010035