Quantitative analysis of Gd@C.sub.82.sub.22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C.sub.82(OH).sub.22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C.sub.82(OH).sub.22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled pl...

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Published inAnalytical and bioanalytical chemistry Vol. 407; no. 9; p. 2383
Main Authors Zheng, Ling-Na, Wang, Meng, Zhao, Lei-Chao, Sun, Bao-Yun, Wang, Bing, Chen, Han-Qing, Zhao, Yu-Liang, Chai, Zhi-Fang, Feng, Wei-Yue
Format Journal Article
LanguageEnglish
Published Springer 01.03.2015
Subjects
Chemical properties
Cisplatin
Identification and classification
Mass spectrometry
Methods
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Summary:Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C.sub.82(OH).sub.22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C.sub.82(OH).sub.22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C.sub.82(OH).sub.22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-014-8422-3