Association of the bovine growth hormone gene with Holstein cattle reproductive parameters/Asociacion del gen de la hormona de crecimiento bovino con parametros reproductivos en ganado Holstein

Objective. To determine the association of intron 3 polymorphism of the bovine growth hormone (BGH) gene, with age at first service, first birth, first postpartum service and second birth in a population of Holstein cows in the state of Antioquia. Materials and methods. The study was conducted on 40...

Full description

Saved in:
Bibliographic Details
Published inRevista MVZ (Medicina Veterinaria y Zootecnia) Vol. 19; no. 3; p. 4249
Main Authors Arango, Juliana G, Echeverri, Julian Z, Lopez, Albeiro H
Format Journal Article
LanguageSpanish
Published Universidad de Cordoba 01.09.2014
Online AccessGet full text

Cover

Loading…
More Information
Summary:Objective. To determine the association of intron 3 polymorphism of the bovine growth hormone (BGH) gene, with age at first service, first birth, first postpartum service and second birth in a population of Holstein cows in the state of Antioquia. Materials and methods. The study was conducted on 408 Holstein cows in 8 herds. Genotyping was performed using PCR-RFLP technique with DNA extracted from peripheral blood by the salting out technique. The Phenotypic information used was collected for 4 years, from a dairy production control program. To determine the association between characteristics and gene polymorphism, parametric statistical analyzes were performed as generalized linear models and linear regression analysis. Results. Allele frequency (positive) was 0.91 and was 0.09 for (negative) allele. Genotypic frequencies were 0.77, 0.2 and 0.03 for (positive/positive), (positive/negative) and (negative/negative) respectively. There were significant differences between the mean of age at first service, age at first birth, age at first postpartum service and age at second birth. For all of the (negative/negative) genotype characteristics there were greater ages for each event. Conclusions. These results suggest that intron-3 polymorphism of the BGH gene is associated with reproductive traits, facilitating the selection of individuals with favorable genotypes for use in breeding programs. Key words: Animal reproduction, molecular markers, PCR-RFLP (Source: Mesh). Objetivo. Determinar la asociacion del polimorfismo del intron 3 del gen de la hormona de crecimiento bovino (BGH) con las edades al primer servicio, primer parto, primer servicio posparto y segundo parto en una poblacion de vacas Holstein del departamento de Antioquia. Materiales y metodos. El estudio se realizo con 408 vacas Holstein ubicadas en 8 hatos. La genotipificacion se llevo a cabo usando la tecnica de PCR-RFLP con ADN extraido de sangre periferica mediante la tecnica de salting out. La informacion fenotipica utilizada fue recopilada durante 4 anos, a partir de un programa de control de produccion lechera. Para determinar la asociacion entre las caracteristicas y el polimorfismo del gen, se realizaron analisis estadisticos parametricos mediante modelos lineales generalizados y analisis de regresion lineal. Resultados. La frecuencia para el alelo (positivo) fue de 0.91 y para el alelo (negativo) fue de 0.09. Las frecuencias genotipicas fueron 0.77, 0.2 y 0.03 para (positivo/positivo), (positivo/negativo) y (negativo/negativo) respectivamente. Se presentaron diferencias significativas entre las medias de la edad al primer servicio, la edad al primer parto, edad al primer servicio postparto y edad al segundo parto. Para todas las caracteristicas el genotipo (negativo/negativo) presento edades mas tardias para cada eventos. Conclusiones. Estos resultados sugieren que el polimorfismo del intron 3 del gen BGH, esta asociado con caracteristicas de tipo reproductivo, facilitando la implementacion de un programa de seleccion de individuos con genotipos favorables, para su uso en programas de mejoramiento genetico animal. Palabras clave: Marcadores moleculares, PCR-RFLP, reproduccion animal (Fuente: Mesh).
ISSN:0122-0268