STATI inhibits STAT3 activation in esophageal squamous cell carcinoma

Background: Signal transducer and activator of transcription (STAT) 1 is an important transcription factor and has been reported to be a tumor suppressor in many types of cancer. However, another STAT family member, STAT3, is considered to be an oncogene. The cross-talk between STAT1 and STAT3 in ca...

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Bibliographic Details
Published inCancer management and research Vol. 10; p. 6517
Main Authors Liu, Zhaoyong, Zhang, Ying, Chen, Yelong, Lin, Youbin, Lin, Zhen, Wang, Hu
Format Journal Article
LanguageEnglish
Published Dove Medical Press Limited 01.01.2018
Subjects
Cancer diagnosis
Carcinoma
Care and treatment
DNA
DNA binding
Esophageal cancer
Gene expression
Genes
Genetic aspects
Immunohistochemistry
Luciferase
Patient outcomes
Squamous cell carcinoma
Tetracyclines
Transcription (Genetics)
Tumors
China
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Summary:Background: Signal transducer and activator of transcription (STAT) 1 is an important transcription factor and has been reported to be a tumor suppressor in many types of cancer. However, another STAT family member, STAT3, is considered to be an oncogene. The cross-talk between STAT1 and STAT3 in cancer has not been fully demonstrated. Materials and methods: Esophageal squamous cell carcinoma (ESCC) was used as a model to examine STAT1-STAT3 cross-regulation in cancer. We detected STAT1-STAT3 binding by co-immunoprecipitation (co-IP) and measured the transcription activity by using a luciferase reporter gene. DNA binding was detected by a DNA probe. Expression of STAT1 and STAT3 in ESCC was detected by immunohistochemistry. Results: We found that STAT1 attenuated STAT3 activity upon oncostatin M treatment by decreasing STAT3 transcription activity and DNA binding ability of STAT3. Furthermore STAT3 downregulation increased the phosphorylation and transcriptional activation of STAT1. Finally, STAT1 expression and STAT3 expression were negatively correlated in ESCC cases. Conclusion: Altogether, this paper demonstrated STAT1 and STAT3 cross-regulation in ESCC and proposed that STAT3 downregulation andior STAT1 accumulation may be a therapeutic approach to treat ESCC. Keywords: STAT1, STAT3, esophageal squamous cell carcinoma, transcription activity
ISSN:1179-1322
1179-1322
DOI:10.2147/CMAR.S182105