Gene Expression Profiles in Cumulus Cells in Poor Responders Undergoing Ovarian Stimulation

Background: Although several studies have investigated the effect of controlled ovarian hyperstimulation on oocytes gene expression, the optimal protocol for patients with a poor ovarian response (POR) has still been a challenge. This study aimed to compare the gene expression profile in human cumul...

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Bibliographic Details
Published inIranian journal of medical sciences Vol. 48; no. S1; p. 65
Main Authors Hosseinishenatal, Shirzad, Mohammadi, Reza, Ahmadi, Hamid, Shirian, Sadegh, Salahi, Elnaz, Eslami, Mojtaba, Sobhani, Aligholi
Format Journal Article
LanguageEnglish
Published Shiraz University of Medical Sciences 01.01.2023
Subjects
Bone morphogenetic proteins
Gene expression
Genes
Genetic research
Leukemia
Pituitary hormones
Iran
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Summary:Background: Although several studies have investigated the effect of controlled ovarian hyperstimulation on oocytes gene expression, the optimal protocol for patients with a poor ovarian response (POR) has still been a challenge. This study aimed to compare the gene expression profile in human cumulus cells (CC) in terms of oocyte maturity in PORs to controlled ovarian hyperstimulation (COH) using delayed start (DS) Gonadotropin-releasing hormone (GnRH ) antagonist versus Flare-up (FU) GnRH agonist. Methods: This prospective randomized controlled study was conducted on 42 women, aged 34.5[+ or -]3.64, who had POR according to bologna criteria. They were randomly divided into two groups, including the FU and the DS group. The growth differentiation factor 9 (GDF9), Bone morphogenetic protein 1 (BMP15), and leukemia inhibitory factor (LIF) gene expression levels in human CC and in vitro fertilization (IVF) outcomes were compared between groups. Results: The mean number of immature, mature, and postmature oocytes was significantly different across the two groups. The gene expression level of GDF9 in immature oocytes was significantly up-regulated in the FU group compared to the DS group (p=0.028). While, there was no significant difference in terms of the gene expression level of LIF and BMP15 in both mature and immature oocytes between the two groups. A negative significant correlation between the LIF gene expression and AFC was observed in the FU group. Conclusion: We concluded that the expression of the GDF9 gene of CC is up-regulated in FU compared to the DS approach. Finally, there is no significant difference between the FU protocol versus DS protocol. Keywords * Ovarian hyperstimulation syndrome * Growth differentiation factor 9 * Leukemia inhibitory factor * Cumulus cells * Ovulation induction * Female
ISSN:0253-0716
1735-3688