A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil/Estudo retrospectivo sobre o diagnostico de mionecroses clostridiais em ruminantes no Brasil

A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) s...

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Published inCiência rural Vol. 47; no. 1
Main Authors Pires, Prhiscylla Sadana, Ecco, Roselene, Silva, Rodrigo Otavio Silveira, de Araujo, Marina Rios, Salvarani, Felipe Masiero, Heneine, Luiz Guilherme Dias, de Oliveira, Carlos Augusto, Jr, Lobato, Francisco Carlos Faria
Format Journal Article
LanguageEnglish
Published Universidade Federal de Santa Maria 01.01.2017
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Summary:A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) samples tested. Clostridium chauvoei and Clostridium perfringens type A were the most common agents found alone, being detected in ten (26.3%) and six (15.8%) samples, respectively. The other cases showed an association of two or three clostridia, with C. perfringens type A detected in 11 (29%) cases. Based on the findings of the present study, polyvalent vaccines against clostridial infections of animals incorporating C. perfringens would be more adequate for preventative purposes in the endemic areas. Key words: gas gangrene, blackleg, malignant edema, cattle, sheep, immunohistochemistry. Imuno-istoquimica padronizada para avaliar o diagnostico etiologico de mionecrose por agentes do genero Clostridium foi utilizada em 38 tecidos formalizados de ruminantes com suspeita clinica e macroscopica, alem de histopatologia compativel com carbunculo sintomatico ou gangrena gasosa. O diagnostico de mionecrose foi confirmado em 37 das 38 (97,4%) amostras avaliadas. Clostridium chauvoei e Clostridium perfringens tipo A foram os unicos agentes encontrados sozinhos, sendo detectados em dez (26,3%) e seis (15,8%) amostras, respectivamente. Os outros casos foram causados por combinacoes de dois ou mais agentes, sendo que C. perfringens type A foi detectado em dez (29,9%) dessas amostras. Baseado nos resultados obtidos, sugere-se que vacinas polivalentes contendo C. perfringens seriam mais adequadas para prevencao de mionecrose causada por clostridios. Palavras-chave: gangrena gasosa, carbunculo sintomatico, edema maligno, bovinos, ovinos, imuno-istoquimica.
ISSN:0103-8478
1678-4596
DOI:10.1590/0103-8478cr20160492