Downregulated expression of PBRM1 in sarcomatoid hepatocellular carcinoma

Sarcomatoid hepatocellular carcinoma (SHCC) is a rare and highly lethal subtype of HCC. The present study aimed to explore the unique markers of SHCC using whole gene expression analysis. Subsequently, gene expression analysis was performed using five sarcomatoid and seven carcinomatoid components o...

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Published inOncology letters Vol. 27; no. 3; p. 1
Main Authors Yoshida, Terufumi, Sakai, Kazuko, Kaibori, Masaki, Ishida, Mitsuaki, Tanaka, Shogo, Kubo, Shoji, Nakai, Takuya, Velasco, Marco A. De, Matsushima, Hideyuki, Tsuta, Koji, Sekimoto, Mitsugu, Nishio, Kazuto
Format Journal Article
LanguageEnglish
Published Spandidos Publications 01.03.2024
Subjects
Chromatin
DNA replication
Ethylenediaminetetraacetic acid
Gene expression
Genes
Hepatoma
Immunohistochemistry
Methylation
Scientific equipment and supplies industry
United States
Japan
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Summary:Sarcomatoid hepatocellular carcinoma (SHCC) is a rare and highly lethal subtype of HCC. The present study aimed to explore the unique markers of SHCC using whole gene expression analysis. Subsequently, gene expression analysis was performed using five sarcomatoid and seven carcinomatoid components of seven tissues from patients with SHCC. The results demonstrated a significant down-regulation of polybromo 1 (PBRM1) gene expression in the sarcomatoid components. Immunohistochemical staining also indicated a decreased expression of PBRM1 in the sarcomatoid components. Moreover, gene ontology enrichment analysis revealed that most of the 336 differentially expressed genes between the sarcomatoid and carcinomatoid components were involved in functions associated with DNA replication and histone methylation, which was consistent with the loss of function of PBRM1 which encodes Switch/sucrose-non-fermentable chromatin remodeling complex protein. Therefore, the results of the present study suggested that PBRM1 may be a candidate biomarker for the evaluation of SHCC. Key words: sarcomatoid hepatocellular carcinoma, polybromo 1, gene ontology, DNA methylation, DNA replication
ISSN:1792-1074
DOI:10.3892/01.2024.14257