Mechanism for Utilization of the IPopulus/I-Derived Metabolite Salicin by a IPseudomonas/I—IRahnella/I Co-Culture

Pseudomonas fluorescens GM16 associates with Populus, a model plant in biofuel production. Populus releases abundant phenolic glycosides such as salicin, but P. fluorescens GM16 cannot utilize salicin, whereas Pseudomonas strains are known to utilize compounds similar to the aglycone moiety of salic...

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Bibliographic Details
Published inMetabolites Vol. 13; no. 2
Main Authors Dahal, Sanjeev, Hurst, Gregory B, Chourey, Karuna, Engle, Nancy L, Burdick, Leah H, Morrell-Falvey, Jennifer L, Tschaplinski, Timothy J, Doktycz, Mitchel J, Pelletier, Dale A
Format Journal Article
LanguageEnglish
Published MDPI AG 01.01.2023
Subjects
Chemical properties
Enterobacter
Enterobacteriaceae
Environmental aspects
Glycosides
Microbiological research
Physiological aspects
Plant metabolites
Poplar
Pseudomonas
United States
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Summary:Pseudomonas fluorescens GM16 associates with Populus, a model plant in biofuel production. Populus releases abundant phenolic glycosides such as salicin, but P. fluorescens GM16 cannot utilize salicin, whereas Pseudomonas strains are known to utilize compounds similar to the aglycone moiety of salicin–salicyl alcohol. We propose that the association of Pseudomonas to Populus is mediated by another organism (such as Rahnella aquatilis OV744) that degrades the glucosyl group of salicin. In this study, we demonstrate that in the Rahnella–Pseudomonas salicin co-culture model, Rahnella grows by degrading salicin to glucose 6-phosphate and salicyl alcohol which is secreted out and is subsequently utilized by P. fluorescens GM16 for its growth. Using various quantitative approaches, we elucidate the individual pathways for salicin and salicyl alcohol metabolism present in Rahnella and Pseudomonas, respectively. Furthermore, we were able to establish that the salicyl alcohol cross-feeding interaction between the two strains on salicin medium is carried out through the combination of their respective individual pathways. The research presents one of the potential advantages of salicyl alcohol release by strains such as Rahnella, and how phenolic glycosides could be involved in attracting multiple types of bacteria into the Populus microbiome.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo13020140