The 15d-[PGJ.sub.2] hydrogel ameliorates atopic dermatitis through suppression of the immune response

The present study examined the efficacy of the topical 15d-[PGJ.sub.2]-poloxamer 407 hydrogel in an atopic dermatitis (AD) animal model. The 15d-[PGJ.sub.2] hydrogel was prepared and characterized. The examined rats possessed AD-Like cutaneous lesions, which were induced using 2,4-dinitrochlorobenze...

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Published inMolecular medicine reports Vol. 19; no. 6; p. 4536
Main Authors Napimoga, Marcelo H, Clemente-Napimoga, Juliana T, Machabanski, Nina M, Juliani, Maria Eduarda A, Acras, Pedro Henrique B.C, Macedo, Cristina G, Abdalla, Henrique B, De Pinho, Antonio Jose, Jr, Soares, Andresa B, Sperandio, Marcelo, De Araujo, Daniele R
Format Journal Article
LanguageEnglish
Published Spandidos Publications 01.06.2019
Subjects
Advertising executives
Allergy
Analysis
Atopic dermatitis
Dermatitis
EDTA
Immune response
Immunoglobulin E
Immunohistochemistry
Skin
Tacrolimus
Tumor necrosis factor
Brazil
United Kingdom
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Summary:The present study examined the efficacy of the topical 15d-[PGJ.sub.2]-poloxamer 407 hydrogel in an atopic dermatitis (AD) animal model. The 15d-[PGJ.sub.2] hydrogel was prepared and characterized. The examined rats possessed AD-Like cutaneous lesions, which were induced using 2,4-dinitrochlorobenzene, the rats were then treated with a hydrogel vehicle, 15d-[PGJ.sub.2] hydrogel or tacrolimus for 14 days. The rats were sacrificed and blood samples were collected to quantify the IgE levels. Subsequently, skin biopsies were stained with toluidine blue to identify mast cells and immunohistochemistry was performed for ROR-[gamma]t and TNF-[alpha]. Histological analyses demonstrated that 15d-[PGJ.sub.2] hydrogel significantly decreased mast cell infiltration (P<0.05) when compared with the AD-group. Tacrolimus at 0.1% exhibited decreased mast cell infiltration; however, this difference was not statistically significant from the AD-group. Topical 15d-[PGJ.sub.2] hydrogel and Tacrolimus 0.1% significantly reduced the serum levels of IgE (P<0.05) compared with the AD-group. Immunohistochemistry revealed a significant decrease in ROR-[gamma]t and TNF-[alpha] positive cell expression (P<0.05) in the 15d-[PGJ.sub.2] hydrogel group compared with the AD-group. In summary, topical administration of 15d-[PGJ.sub.2] hydrogel had a beneficial effect on AD symptoms, suggesting that this formulation may be a useful strategy for the treatment of AD. Key words: 15d-[PGJ.sub.2], inflammation, atopic dermatitis, immunology, allergy
ISSN:1791-2997
1791-3004
DOI:10.3892/mmr.2019.10156