Frequency of Clustered Regularly Interspaced Short Palindromic Repeats em cepas nao-clinicas de Enterococcus faecalis e Enterococcus faecium

• Published in: Brazilian journal of biology Vol. 79; no. 3; p. 460
• Main Authors: Huescas, C.G.Y, Pereira, R.I, Prichula, J, Azevedo, P.A, Frazzon, J, Frazzon, A.P.G
• Format: Journal Article
• Language: English
• Published: Association of the Brazilian Journal of Biology 01.07.2019
• Subjects: Brasil
• ISSN: 1519-6984; 1678-4375
• DOI: 10.1590/1519-6984.183375

The fidelity of the genomes is defended by mechanism known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems. Three Type II CRISPR systems (CRISPR1-cas, CRISPR2 and CRISPR3-cas) have been identified in enterococci isolates from clinical and environmental samples. The aim of this study was to observe the distribution of CRISPR1-cas, CRISPR2 and CRISPR3-cas in non-clinical strains of Enterococcus faecalis and Enterococcus faecium isolates from food and fecal samples, including wild marine animals. The presence of CRISPRs was evaluated by PCR in 120 enterococci strains, 67 E. faecalis and 53 E. faecium. It is the first report of the presence of the CRISPRs system in E. faecalis and E. faecium strains isolated from wild marine animal fecal samples. The results showed that in non-clinical strains, the CRISPRs were more frequently detected in E. faecalis than in E. faecium. And the frequencies of CRISPR1-cas and CRISPR2 were higher (60%) in E. faecalis strains isolated from animal feces, compared to food samples. Both strains showed low frequencies of CRISPR3-cas (8.95% and 1.88%). In conclusion, the differences in the habitats of enterococcal species may be related with the results observe in distribution of CRISPRs systems. Keywords: Enterococcus faecalis, Enterococcus faecium, CRISPRs, food samples, fecal samples, wild marine animals. A fidelidade dos genomas e defendida por mecanismos conhecidos como sistemas de repeticoes palindromicas curtas agrupadas e regularmente interespacadas (CRISPRs). Tres tipos de sistemas CRISPR II (CRISPR1-cas, CRISPR2 e CRISPR3-cas) tem sido identificados em cepas de enterococos isolados de amostras clinicas e ambientais. O objetivo deste estudo foi observar a distribuicao dos CRISPR1-cas, CRISPR2 e CRISPR3-cas em cepas nao-clinicas de Enterococcus faecalis e Enterococcus faecium isoladas de amostras alimenticias e fecais, incluindo animais marinhos selvagens. A presenca dos CRISPRs foi determinada por PCR em 120 cepas de enterococos, sendo 67 E. faecalis e 53 E. faecium. E o primeiro relato da presenca do sistema CRISPRs nas estirpes E. faecalis e E. faecium isoladas de amostras fecais de animais marinhos selvagens. Os resultados mostraram que em cepas nao-clinicas, os CRISPRs foram mais frequentemente detectados em E. faecalis do que em E. faecium. E as frequencias de CRISPR1-cas e CRISPR2 foram maiores (60%) em cepas de E. faecalis isoladas de fezes de animais, quando comparadas a amostras de alimentos. Ambas as cepas apresentaram baixas frequencias de CRISPR3-cas (8,95% e 1,88%). Em conclusao, as diferencas nos habitats das especies de enterococos podem estar relacionadas com os resultados observados na distribuicao dos sistemas CRISPRs. Palavras-chave: Enterococcus faecalis, Enterococcus faecium, CRISPRs, amostras alimentares, amostras fecais, animais marinhos selvagens.