Fluorometric methods for determination of H.sub.2O.sub.2, glucose and cholesterol by using MnO.sub.2 nanosheets modified with 5-carboxyfluorescein

A fluorescent probe for H.sub.2O.sub.2 is described. It is composed of MnO.sub.2 nanosheets and 5-carboxyfluorescein and was characterized by fluorescence, transmission electron microscopy, ultraviolet-visible absorption spectra, energy dispersive X-ray and Fourier transform infrared spectroscopy. T...

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Bibliographic Details
Published inMikrochimica acta (1966) Vol. 186; no. 5
Main Authors Han, Ting, Zhu, Shengmei, Wang, Sicheng, Wang, Baojuan, Zhang, Xiaojun, Wang, Guangfeng
Format Journal Article
LanguageEnglish
Published Springer 01.05.2019
Subjects
Analysis
Cholesterol
Dextrose
Fluorescence microscopy
Glucose
Infrared spectroscopy
Methods
Oxidases
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Summary:A fluorescent probe for H.sub.2O.sub.2 is described. It is composed of MnO.sub.2 nanosheets and 5-carboxyfluorescein and was characterized by fluorescence, transmission electron microscopy, ultraviolet-visible absorption spectra, energy dispersive X-ray and Fourier transform infrared spectroscopy. The probe, with fluorescence excitation/emission maxima at 490/518 nm, responds to H.sub.2O.sub.2 in the 1 to 200 [mu]M concentration range and has a 0.33 [mu]M detection limit. The probe was used in enzymatic assays for glucose and cholesterol by using the respective oxidases which produce H.sub.2O.sub.2. Responses are linear in the concentration range from 0.5 to 200 [mu]M in case of glucose, and from 1 to 300 [mu]M in case of cholesterol. The method was applied to quantify glucose and cholesterol in (spiked) serum samples.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-019-3381-1