Long Noncoding RNA FGD5-ASI Promotes Glioma Cell Proliferation, Migration and Invasion by Regulating wnt/[beta]-Catenin Pathway
Purpose: To investigate the specific function of long noncoding RNA FGD5 antisense RNA 1 (lncRNA FGD5-AS1) in glioma. Materials and Methods: The level of FGD5-AS1 was detected in clinical samples and cell lines by qRT-PCR. Small interfering RNA (siRNA) of FGD5-AS1 or scramble siRNA was transfected i...
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Published in | Cancer management and research Vol. 12; p. 6187 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dove Medical Press Limited
31.07.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Purpose: To investigate the specific function of long noncoding RNA FGD5 antisense RNA 1 (lncRNA FGD5-AS1) in glioma. Materials and Methods: The level of FGD5-AS1 was detected in clinical samples and cell lines by qRT-PCR. Small interfering RNA (siRNA) of FGD5-AS1 or scramble siRNA was transfected into U87 cell lines to examine the role of FGD5-AS1 on glioma development. The proliferation of glioma cells was tested by Cell Counting Kit-8 (CCK-8), the migration and invasion of glioma cells were tested by transwell assay without matrigel or with matrigel. Western blot was used to detect the protein expression, and XAV-939 was used to inhibit wnt/[beta]-catenin pathway. The effect of FGD5-AS1 on tumorigenesis of glioma was confirmed by xenograft nude mice model. Results: FGD5-AS1 was significantly increased in glioma tissues and cells. Loss of FGD5-AS1 inhibited the proliferation, migration and invasion of U87 cells. Furthermore, over-expression of FGD5-AS1 increased the mRNA and protein levels of [beta]-catenin and cyclin D1. Blocking of wnt/[beta]-catenin using XAV-939 reversed the promotion role of FGD3-AS1 on glioma cells' migration and invasion. The in vivo tumor growth assay showed that FGD3-AS1 accelerated glioma tumorigenesis with activating wnt/[beta]-catenin pathway. Conclusion: Our research emphasized FGD5-AS1 acting as an oncogene by regulating wnt/[beta]-catenin signaling pathway, thus providing some novel experimental basis for clinical treatment of glioma. Keywords: lncRNA FGD5-AS1, glioma, cell proliferation, migration, wnt/[beta]-catenin pathway |
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ISSN: | 1179-1322 1179-1322 |
DOI: | 10.2147/CMAR.S250284 |