A different approach to diagnosis of subclinical mastitis: milk arginase activity/Dijagnostika supklinickog mastitisa zasnovana na aktivnosti arginaze u mlijeku

• Published in: Veterinarski arhiv Vol. 83; no. 6; p. 603
• Main Authors: Kandemir, Fatih M, Yuksel, Murat, Ozdemir, Necmi, Deveci, Huseyin
• Format: Journal Article
• Language: English
• Published: Sveuciliste U Zagrebu 01.11.2013
• Subjects: Animals; Arginase; Dairy cattle; Diagnosis; Diseases; Health aspects; Mastitis; United States
• ISSN: 0372-5480; 1331-8055

The aim of this study was to determine the relationships between subclinical mastitis and milk arginase activity in dairy cows. Thirty, various breed 4-8 year old cows from Firat University Animal Hospital were used in the study. Subclinical mastitis was diagnosed by the California Mastitis Test (CMT)) combined with bacteriological examination of milk samples. The subclinical mastitis group consisted of fifteen clinically healthy butt CMT and microbiologically positive animals. CMT and microbiologically negative animals ((n = 15) served as the control group. Arginase activity in milk samples was measured by spectrophotometer using the thiocemicarbazide diacetylmonoxime urea (TDMU) method and protein was determined with the folin phenol reagent Arginase activity in the milk of animals with subclinical mastitis (0.26 [+ or -] 0.07 U/mg protein) significantly increased compared to the arginase activity in the milk from healthy animals in the control group (0.10 [+ or -] 0.03 U/mg protein). Milk enzyme activity in the mildly severe disease (0.26 [+ or -] 0.01 U/mg protein) was significantly lower than in the moderately severe disease (0.30 [+ or -] 0.01 U/mg). No relationship was found between the nature of the bacterial infection of the cows with subclinical mastitis and their milk arginase activity. It was concluded that assay of milk arginase activity may be used as an additional laboratory method for the diagnosis off subclinical mastitis. Key words: subclinical mastitis, arginase, cow, California mastitis test Cilj istrazivanja bio je utvrditi povezanost izmedu supklinickog mastitisa i aktivnosti arginaze u mlijeku krava. U istrazivanje je ukljuceno 30 krava u dobi od 4 do 8 godina. Krave su bile razlicitih pasmina i pacijenti bolnice Firat Sveucilista. Supklinicki mastitis dijagnosticiran je kombinacijom kalifornijskog mastitis testa (KMT) i bakterioloske pretrage uzoraka mlijeka. Skupinu sa supklinickim mastitisom cinilo je 15 klinicki zdravih krava pozitivnih pretragom s KMT i bakterioloski pozitivnim nalazom. Petnaest krava s pozitivnim KMT i bakterioloski negativnim nalazom posluzilo je kao kontrolna skupina. Aktivnost arginaze u uzorcima mlijeka utvrdena je spektrofotometrijski, uz primjenu tiocemikarbazid diacetilmonoksim ureja metode (TDMU), a bjelancevine su bile odredene pomocu folin fenol reagensa. Aktivnost arginaze u mlijeku krava sa supklinickim mastitisom (0,26 [+ or -] 0,07 U/mg bjelancevina) bila je znacajno povisena u usporedbi s uzorcima iz mlijeka zdravih krava kontrolne skupine (0,10 [+ or -] 0,03 U/mg bjelancevina). U krava sa slabo izrazenim simptomima bolesti, aktivnost enzima u mlijeku (0,26 [+ or -] 0,01 U/mg protein) bila je znacajno niza u odnosu na krave s umjerenim simptomima (0,30 [+ or -] 0,01 U/mg). Nije utvrdena povezanost izmedu prirode bakterijske infekcije i supklinickog mastitisa odnosno aktivnosti arginaze u mlijeku. Zakljucak je da analiza aktivnosti arginaze moze posluziti kao dodatna laboratorijska metoda za dijagnostiku supklinickog mastitisa. Kljucne rijeci: supklinicki mastitis, arginaza, krave, kalifornijski mastitis test