Anti-Inflammatory Effect of IPterospartum tridentatum/I Leaf Extract in Acute and Chronic Inflammation

• Published in: Applied sciences Vol. 13; no. 7
• Main Authors: Laranjeira, Inês Ma, Gonçalves, João N. D, Gonçalves, Cátia, Silva, Marlene, Mouta, Nuno, Dias, Alberto C. P, Pinto-Ribeiro, Filipa
• Format: Journal Article
• Language: English
• Published: MDPI AG 01.04.2023
• Subjects: Anti-inflammatory drugs; High performance liquid chromatography; Inflammation; Isoflavones; Medicine, Botanic; Medicine, Herbal; Osteoarthritis; Pharmaceutical industry; Protein binding; United Kingdom; United States; Portugal; Germany; Spain
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app13074494

Pterospartum tridendatum (PtL) is used in popular medicine for its anti-inflammatory properties. Herein, we show PtL extract reversed experimental osteoarthritis induced knee edema, as well as acute ear edema, highlighting its potential as an adjuvant in the management of inflammation. Pterospartum tridendatum is an important source of active compounds with anti-inflammatory properties. The ability of P. tridentatum leaves methanolic extract in preventing/reversing inflammation was studied in adult rats using a model of experimental osteoarthritis (OA) and ear edema. Control animals (SHAM) were administered phosphate buffer solution (PBS), while OA animals received either P. tridentatum 100 mg/kg, 300 mg/kg, or a commercial anti-inflammatory (15 mg/Kg, Ibuprofen) via gavage, daily, for three weeks. Ear edema was induced, and the animals were divided into five groups treated with: (i) ethanol, (ii) P. tridentatum, (iii) croton oil, (iv) croton oil + P. tridentatum, and (v) croton oil + medrol. The inflammatory effect was evaluated by the measurement of the knee and ear edema. The chromatographic profile, evaluated by HPLC-DAD, showed numerous phenolic compounds are present. In the docking analysis of these compounds, isoquercetin demonstrated strong molecular interactions for peroxisome proliferator-activated receptor alpha and gamma (PPARα and PPARƴ, respectively), protein kinase 2 subunit α (CK2 α), and 5-lipoxygenase-activating proteins. Genistein had strong docking binding energies for CK2α and prostaglandin H (2) synthase-1. Our analysis showed the treatment with P. tridentatum extract reversed OA-induced edema in the rat knee, as well as ear edema, highlights this plant as a potential source of compounds that can be used as adjuvants in the management of inflammation.