Sialochemistry in patients with oral lichen planus/Sijalokemijski nalaz za bolesnike s oralnim lihen planusom

• Published in: Acta stomatologica croatica Vol. 45; no. 3; p. 184
• Main Authors: Boras, Vanja Vucicevic, Rogic, Dunja, Brailo, Vlaho, Vidovic-Juras, Danica, Glazar, Irena, Muhvic-Urek, Miranda
• Format: Journal Article
• Language: English
• Published: Sveuciliste U Zagrebu 01.09.2011
• Subjects: Autoimmune diseases; Care and treatment; Dental care; Dental hygiene; Development and progression; Health aspects; Mouth; Physiological aspects; Croatia
• ISSN: 0001-7019; 1846-0410

Objective: It is well known that certain salivary constituents might be disturbed in patients suffering from oral lichen planus (OLP), however, the results of the published studies are inconsistent. The aim of this study was to assess the concentrations of salivary analytes because most of them are part of salivary enzymes which maintain integrity of the oral mucosa which is compromised in OLP patients. Materials and Methods: In 25 patients with OLP (73±1.4 yrs) and in the 24 controls (24±3.7yrs) levels of total proteins, amylase, salivary magnesium, calcium, copper, chloride, phosphate, potassium and sodium were determined. Total proteins were determined by pyrogalol colorimetric method. Amylase levels were determined by continued colorimetric method. Salivary sodium, potassium and chloride were determined by indirect potentiometry whereas salivary copper and magnesium were determined by atomic absorption spectrophotometry whereas phosphates were determined by colorimetric method with use of molybdate. Statistical analysis was performed by use of [chi square] test, Mann Whitney U test analysis of covariance and Spearman's correlation. Results: Significantly higher concentration of salivary chloride was detected in OLP patients in comparison to the controls (p=0.025). Furthermore, when the obtained results for salivary analytes were adjusted with respect to the salivary flow rate, increased levels of salivary potassium, sodium, chloride and total proteins were found in patients with OLP when compared to the controls (p=0.622; p=0.504; p=0.600; p=0.586). Concentrations of salivary analytes were not affected by smoking habit. Conclusions: Increased levels of salivary sodium, potassium and chloride are probably a result of significant differences in salivary flow rate between patients with OLP and controls and do not indicate increased salivary antimicrobial activity. Key words Saliva; Lichen Planus, Oral; Salivary Proteins and Peptides Svrha: Zna se da odredeni sastojci sline mogu biti poremeceni kod bolesnika koji pate od oralnoga lichen planusa (OLP-a), no rezultati objavljenih istrazivanja nisu konzistentni. Svrha ovog istrazivanja bila je odrediti koncentraciju salivarnih analita u sklopu salivarnih enzima koji odrzavaju integritet oralnih sluznica, a on je ostecen kod oboljelih od oralnoga lichen planusa. Ispitanici i postupci: Skupini od 25 bolesnika s OLP-om (73±1,4 godine) i 24 kontrolna ispitanika (24±3,7 godina) odredeni su u slini ukupni proteini, amilaza, magnezij, kalcij, bakar, klorid, fosfat i kalij. Ukupni proteini odredeni su kolorimetrijskom metodom uz pirogalol. Natrij, kalij i klorid izmjereni su indirektnom potenciometrijom, bakar i magnezij atomskom apsorpcijskom spektrofotometrijom, a fosfat kolorimetrijskom uz pomoc molibdata. Statisticka analiza obavljena je uz pomoc [chi square] testa, Mann-Whitneyeva U-testa, analizom kovarijance i Spearmanovom korelacijom. Rezultati: Znatno povisene vrijednosti salivarnog klorida pronadene su kod oboljelih od OLP-a u odnosu prema sudionicima u kontrolnoj skupini (p=0,025). Nakon toga, kada su dobiveni rezultati prilagodeni s obzirom na kolicinu izlucene sline, ustanovljene su povisene vrije dnosti salivarnog kalija, natrija, klorida i ukupnih proteina kod oboljelih od oralnoga lichen planusa u odnosu prema kontrolnoj skupini (p=0,622; p=0,504; p=0,00; p=0,586). Koncentracija salivarnih analita nije bila povezana s pusenjem. Zakljucak: Povisene vrijednosti salivarnog natrija, kalija i klorida vjerojatno su posljedica velikih razlika u kolicini izlucene sline izmedu oboljelih od OLP-a i sudionika u kontrolnoj skupini i ne upucuju na pojacanu antimikrobnu aktivnost kod bolesnika s OLP-om. Kljucne rijeci slina; lihen planus; proteini i peptidi sline