Grafen Oksitin Sentezlenmesi ve Sitotoksik Etkisinin in vitro Olarak Degerlendirilmesi/Synthesis of Graphene Oxide and in vitro Evaluation of Its Cytotoxic Effect

• Published in: Journal of academic research in medicine Vol. 13; no. 2; p. 58
• Main Authors: zsobaci, Nural Pastaci, Ergün, Dilek Düzgün
• Format: Journal Article
• Language: English
• Published: Galenos Yayinevi Tic. Ltd 01.08.2023
• Subjects: Biomedical engineering; Graphene; Health aspects; Methods; Physiological aspects; Turkey
• ISSN: 2146-6505
• DOI: 10.4274/jarem.galenos.2023.78300

Amaç: Karbon bazli bir malzeme olan grafenoksit, sahip oldugu fiziksel ve kimyasal özellikler nedeniyle hücre etiketleme, hücre görüntüleme, biyosensörler, ilaç salinimi çalismalari gibi birçok biyomedikal uygulama için potansiyel bir aday olarak düsünülmektedir. Grafen oksitin sentezlenmesi birden fazla yöntemle gerçeklesebilmektedir. Bu çalismada sentezledigimiz grafenoksit numunelerinin sitotoksik etkilerinin degerlendirilmesi amaçlamistir. Yöntemler: Grafen oksitin sentezlenmesi Hummers yöntemiyle yapilmistir. Grafen oksitin sitotoksik etkileri 3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazolyum bromür testi ile insan embriyonik börek hücrelerinde (HEK293) degerlendirilmistir. Yirmi dört saat, 48 saat ve 72 saatlik farkli dozlarda grafen oksit inkübasyonunun ardindan hücrelerin %50 canlilik gösterdigi I[C.sub.50] degerleri hesaplanmistir. Bulgular: Yirmi dört saat, 48 saat ve 72 saat sonunda I[C.sub.50] degerleri sirasiyla 206,18 [micro]g/mL, 108,98 [micro]g/mL, 55,54 [micro]g/mL olarak ölçülmüstür. Inkübasyon süresi arttiginda I[C.sub.50] degerinin azaldigi tespit edilmistir. Sonuç: Sentezlenen grafen oksitin sitotoksik etkisinin maruz kalma süresine bagli olarak degisiklik gösterdigi belirlenmistir. Anahtar kelimeler: Grafen, grafen oksit, HEK293, toksisite Objective: Graphen oxide, a carbon-based material, is considered as a potential material for many biomedical applications such as cell labeling, cell imaging, biosensors, drug release studies, due to its physical and chemical properties. Graphene oxide can be synthesized by more than one method. In this study, it was aimed to evaluate the cytotoxic effects of the graphenoxide samples we synthesized. Methods: Synthesis of graphene oxide was done by Hummers method. The cytotoxic effects of graphene oxide were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay in human embryonic kidney cells (HEK293). After 24 hours, 48 hours and 72 hours of graphene oxide incubation at different doses, the I[C.sub.50] values were calculated which 50% percentage of cell viability. Results: After 24 hours, 48 hours and 72 hours, I[C.sub.50] values were measured as 206.18 [micro]g/mL, 108.98 [micro]g/mL, and 55.54 [micro]g/mL, respectively. It was determined that the I[C.sub.50] value decreased as the incubation time increased. Conclusion: It was determined that the cytotoxic effect of the synthesized graphene oxide varies depending on the exposure time. Keywords: Graphene, graphen oxide, HEK293, toxicity