Induction of Apoptosis in BCR-ABL Fusion Associated Chronic Myeloid Leukemia Cells by Camellia kissi Wall. Extract

Objective: Camellia kissi, a prominent tea, lacks academic works. In a previous report, this plant substantially affected chronic myeloid leukemia cells. Understanding the mechanism of action of this tea species on leukemia cells will contribute to researching alternative treatment methods in the co...

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Published inEuropean journal of biology Vol. 83; no. 1; p. 117
Main Authors Xuan, Nguyen Anh, Quan, Nguyen Trung, Ly, Bui Thi Kim, Chi, Hoang Thanh
Format Journal Article
LanguageEnglish
Published Istanbul University Press 01.06.2024
Subjects
Apoptosis
B cells
Cancer
Chronic myeloid leukemia
Drug resistance
Genetic transcription
Methylene blue
Nilotinib
Oncology, Experimental
RNA
Vietnam
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Summary:Objective: Camellia kissi, a prominent tea, lacks academic works. In a previous report, this plant substantially affected chronic myeloid leukemia cells. Understanding the mechanism of action of this tea species on leukemia cells will contribute to researching alternative treatment methods in the context of drug resistance in chronic myeloid leukemia, which is constantly increasing. Materials and Methods: C. kissi comes from Lam Dong, Vietnam. The crude tea extract in methanol was obtained. The flow cytometry method with Annexin V and PI staining and the DNA fragmentation assays were used to indicate the apoptosis cells. The reversed transcription real-time PCR reactions were conducted to measure the mRNA level under the treatment. Results: The results showed the apoptosis-inducing capacity of the C. kissi extract on K562 cells, and the impact was suggested to be through the induction of intracellular apoptosis and cell cycle arrest. Conclusion: The apoptosis induction on K562 caused by C. kissi was reported for the first time. Initial recognition of the signaling pathway of inhibition is through the BCR-ABL/PTEN. Keywords: Camellia kissi, Chronic myeloid leukemia, Leukemia, Gene expression.
ISSN:2602-2575
DOI:10.26650/EurJBiol.2024.1399845