Validation of a Spectrophotometric Method for Urinary Iodine Determination on Microplate Based on Sandell-Kolthoff Reaction
Objective: Iodine is an essential part of the thyroid hormones thyroxine and triiodothyronine. Therefore, it is essential to monitor iodine supply in a population. The biochemical marker for assessing and controlling iodine is urinary iodine concentration (UIC). Materials and Methods: This cross-sec...
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Published in | Laboratory medicine Vol. 53; no. 4; p. 376 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford University Press
01.07.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Objective: Iodine is an essential part of the thyroid hormones thyroxine and triiodothyronine. Therefore, it is essential to monitor iodine supply in a population. The biochemical marker for assessing and controlling iodine is urinary iodine concentration (UIC). Materials and Methods: This cross-sectional study included 180 pregnant women and 308 women of reproductive age. Urine specimens from 185 of the 488 volunteers were used. The urine specimens were measured using 2 methods: (1) ammonium persulfate digestion (APD), followed by the Sandell-Kolthoff (S-K) reaction modified on microplate for spectrophotometric detection; and (2) the reference met6hod, inductively coupled plasma mass spectrometry (ICP-MS). Results: The regression equation between the methods was ICP-MS method = 1.137*(APD S-K)-5.57. A Passing-Bablok regression showed no deviation from linearity (P =.17). A Bland-Altman plot showed a negative mean bias of-2.7%. Conclusion: The APD S-K reaction modified on microplate for spectrophotometric detection of UIC can be implemented into routine work. Its results are comparable to those of laboratories worldwide and to ICP-MS. Keywords: urinary iodine concentration, Sandell-Kolthoff reaction, thyroid, iodine deficiency, spectrophotometric detection |
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ISSN: | 0007-5027 1943-7730 |
DOI: | 10.1093/labmed/Imab117 |