Validation of a Spectrophotometric Method for Urinary Iodine Determination on Microplate Based on Sandell-Kolthoff Reaction

Objective: Iodine is an essential part of the thyroid hormones thyroxine and triiodothyronine. Therefore, it is essential to monitor iodine supply in a population. The biochemical marker for assessing and controlling iodine is urinary iodine concentration (UIC). Materials and Methods: This cross-sec...

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Published inLaboratory medicine Vol. 53; no. 4; p. 376
Main Authors Oblak, Adrijana, Arohonka, Petra, Erlund, Iris, Kuzmanovska, Sonja, Zaletel, Katja, Gaberscek, Simona
Format Journal Article
LanguageEnglish
Published Oxford University Press 01.07.2022
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Summary:Objective: Iodine is an essential part of the thyroid hormones thyroxine and triiodothyronine. Therefore, it is essential to monitor iodine supply in a population. The biochemical marker for assessing and controlling iodine is urinary iodine concentration (UIC). Materials and Methods: This cross-sectional study included 180 pregnant women and 308 women of reproductive age. Urine specimens from 185 of the 488 volunteers were used. The urine specimens were measured using 2 methods: (1) ammonium persulfate digestion (APD), followed by the Sandell-Kolthoff (S-K) reaction modified on microplate for spectrophotometric detection; and (2) the reference met6hod, inductively coupled plasma mass spectrometry (ICP-MS). Results: The regression equation between the methods was ICP-MS method = 1.137*(APD S-K)-5.57. A Passing-Bablok regression showed no deviation from linearity (P =.17). A Bland-Altman plot showed a negative mean bias of-2.7%. Conclusion: The APD S-K reaction modified on microplate for spectrophotometric detection of UIC can be implemented into routine work. Its results are comparable to those of laboratories worldwide and to ICP-MS. Keywords: urinary iodine concentration, Sandell-Kolthoff reaction, thyroid, iodine deficiency, spectrophotometric detection
ISSN:0007-5027
1943-7730
DOI:10.1093/labmed/Imab117